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001     153368
005     20240321221042.0
024 7 _ |a 10.1038/s41590-020-0707-4
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024 7 _ |a pmid:32541832
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024 7 _ |a 1529-2908
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024 7 _ |a 1529-2916
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037 _ _ |a DZNE-2020-01365
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Masuda, Takahiro
|0 P:(DE-HGF)0
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|e Corresponding author
245 _ _ |a Novel Hexb-based tools for studying microglia in the CNS.
260 _ _ |a London
|c 2020
|b Springer Nature Limited
336 7 _ |a article
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336 7 _ |a Journal Article
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336 7 _ |a ARTICLE
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a Journal Article
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520 _ _ |a Microglia and central nervous system (CNS)-associated macrophages (CAMs), such as perivascular and meningeal macrophages, are implicated in virtually all diseases of the CNS. However, little is known about their cell-type-specific roles in the absence of suitable tools that would allow for functional discrimination between the ontogenetically closely related microglia and CAMs. To develop a new microglia gene targeting model, we first applied massively parallel single-cell analyses to compare microglia and CAM signatures during homeostasis and disease and identified hexosaminidase subunit beta (Hexb) as a stably expressed microglia core gene, whereas other microglia core genes were substantially downregulated during pathologies. Next, we generated HexbtdTomato mice to stably monitor microglia behavior in vivo. Finally, the Hexb locus was employed for tamoxifen-inducible Cre-mediated gene manipulation in microglia and for fate mapping of microglia but not CAMs. In sum, we provide valuable new genetic tools to specifically study microglia functions in the CNS.
536 _ _ |a 344 - Clinical and Health Care Research (POF3-344)
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588 _ _ |a Dataset connected to CrossRef, PubMed,
650 _ 7 |a Luminescent Agents
|2 NLM Chemicals
650 _ 7 |a Luminescent Proteins
|2 NLM Chemicals
650 _ 7 |a red fluorescent protein
|2 NLM Chemicals
650 _ 7 |a beta-Hexosaminidase beta Chain
|0 EC 3.2.1.52
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Brain: cytology
|2 MeSH
650 _ 2 |a Brain: immunology
|2 MeSH
650 _ 2 |a Brain: pathology
|2 MeSH
650 _ 2 |a CRISPR-Cas Systems: genetics
|2 MeSH
650 _ 2 |a Encephalomyelitis, Autoimmune, Experimental: immunology
|2 MeSH
650 _ 2 |a Encephalomyelitis, Autoimmune, Experimental: pathology
|2 MeSH
650 _ 2 |a Facial Nerve Injuries: immunology
|2 MeSH
650 _ 2 |a Facial Nerve Injuries: pathology
|2 MeSH
650 _ 2 |a Gene Knock-In Techniques
|2 MeSH
650 _ 2 |a Genes, Reporter: genetics
|2 MeSH
650 _ 2 |a Genetic Loci: genetics
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Intravital Microscopy
|2 MeSH
650 _ 2 |a Luminescent Agents: chemistry
|2 MeSH
650 _ 2 |a Luminescent Proteins: chemistry
|2 MeSH
650 _ 2 |a Luminescent Proteins: genetics
|2 MeSH
650 _ 2 |a Macrophages: immunology
|2 MeSH
650 _ 2 |a Macrophages: metabolism
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Microglia: immunology
|2 MeSH
650 _ 2 |a Microglia: metabolism
|2 MeSH
650 _ 2 |a NIH 3T3 Cells
|2 MeSH
650 _ 2 |a RNA-Seq
|2 MeSH
650 _ 2 |a Single-Cell Analysis
|2 MeSH
650 _ 2 |a Transfection
|2 MeSH
650 _ 2 |a beta-Hexosaminidase beta Chain: genetics
|2 MeSH
650 _ 2 |a beta-Hexosaminidase beta Chain: metabolism
|2 MeSH
700 1 _ |a Amann, Lukas
|0 P:(DE-HGF)0
|b 1
700 1 _ |a Sankowski, Roman
|0 P:(DE-HGF)0
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700 1 _ |a Staszewski, Ori
|0 P:(DE-HGF)0
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700 1 _ |a Lenz, Maximilian
|0 P:(DE-HGF)0
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700 1 _ |a D Errico, Paolo
|0 P:(DE-HGF)0
|b 5
700 1 _ |a Snaidero, Nicolas
|0 P:(DE-HGF)0
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700 1 _ |a Costa Jordão, Marta Joana
|0 P:(DE-HGF)0
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700 1 _ |a Böttcher, Chotima
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700 1 _ |a Kierdorf, Katrin
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700 1 _ |a Jung, Steffen
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700 1 _ |a Priller, Josef
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700 1 _ |a Misgeld, Thomas
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700 1 _ |a Vlachos, Andreas
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700 1 _ |a Meyer-Luehmann, Melanie
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700 1 _ |a Knobeloch, Klaus-Peter
|0 P:(DE-HGF)0
|b 15
700 1 _ |a Prinz, Marco
|0 P:(DE-HGF)0
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773 _ _ |a 10.1038/s41590-020-0707-4
|g Vol. 21, no. 7, p. 802 - 815
|0 PERI:(DE-600)2026412-4
|n 7
|p 802 - 815
|t Nature immunology
|v 21
|y 2020
|x 1529-2916
856 4 _ |u https://pub.dzne.de/record/153368/files/DZNE-2020-01365.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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