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@ARTICLE{Wu:154224,
      author       = {Wu, Yue and Tam, Wing-Sze and Chau, Ho-Fai and Kaur,
                      Simranjeet and Thor, Waygen and Aik, Wei Shen and Chan, Wai
                      Lun and Zweckstetter, Markus and Wong, Ka-Leung},
      title        = {{S}olid-phase fluorescent {BODIPY}–peptide synthesis via
                      in situ dipyrrin construction},
      journal      = {Chemical science},
      volume       = {11},
      number       = {41},
      issn         = {2041-6539},
      address      = {Cambridge},
      publisher    = {RSC},
      reportid     = {DZNE-2021-00085},
      pages        = {11266 - 11273},
      year         = {2020},
      abstract     = {Traditional fluorescent peptide chemical syntheses hinge on
                      the use of limited fluorescent/dye-taggable unnatural amino
                      acids and entail multiple costly purifications. Here we
                      describe a facile and efficient protocol for in situ
                      construction of dipyrrins on the N-terminus with 20 natural
                      and five unnatural amino acids and the lysine's side chain
                      of selected peptides/peptide drugs through Fmoc-based
                      solid-phase peptide synthesis. The new strategy enables the
                      direct formation of boron–dipyrromethene
                      (BODIPY)–peptide conjugates from simple aldehyde and
                      pyrrole derivatives without pre-functionalization, and only
                      requires a single-time chromatographic purification at the
                      final stage. As a model study, synthesized EBNA1-targeting
                      BODIPY1–Pep4 demonstrates intact selectivity in vitro,
                      responsive fluorescence enhancement, and higher light
                      cytotoxicity due to the photo-generation of cytotoxic
                      singlet oxygen. This work offers a novel practical synthetic
                      platform for fluorescent peptides for multifaceted
                      biomedical applications.},
      cin          = {AG Zweckstetter},
      ddc          = {540},
      cid          = {I:(DE-2719)1410001},
      pnm          = {342 - Disease Mechanisms and Model Systems (POF3-342)},
      pid          = {G:(DE-HGF)POF3-342},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34094367},
      pmc          = {pmc:PMC8162834},
      doi          = {10.1039/D0SC04849F},
      url          = {https://pub.dzne.de/record/154224},
}