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@ARTICLE{Mller:154261,
author = {Müller, Franziska E and Schade, Sophie K and Cherkas,
Volodymyr and Stopper, Laura and Breithausen, Björn and
Minge, Daniel and Varbanov, Hristo and Wahl-Schott,
Christian and Antoniuk, Svitlana and Domingos, Catia and
Compan, Valérie and Kirchhoff, Frank and Henneberger,
Christian and Ponimaskin, Evgeni and Zeug, Andre},
title = {{S}erotonin receptor 4 regulates hippocampal astrocyte
morphology and function.},
journal = {Glia},
volume = {69},
number = {4},
issn = {1098-1136},
address = {Bognor Regis [u.a.]},
publisher = {Wiley-Liss},
reportid = {DZNE-2021-00115},
pages = {872 - 889},
year = {2021},
note = {ISSN 1098-1136 not unique: **3 hits**.},
abstract = {Astrocytes are an important component of the multipartite
synapse and crucial for proper neuronal network function.
Although small GTPases of the Rho family are powerful
regulators of cellular morphology, the signaling modules of
Rho-mediated pathways in astrocytes remain enigmatic. Here
we demonstrated that the serotonin receptor 4 (5-HT4 R) is
expressed in hippocampal astrocytes, both in vitro and in
vivo. Through fluorescence microscopy, we established that
5-HT4 R activation triggered RhoA activity via Gα13
-mediated signaling, which boosted filamentous actin
assembly, leading to morphological changes in hippocampal
astrocytes. We investigated the effects of these 5-HT4
R-mediated changes in mixed cultures and in acute slices, in
which 5-HT4 R was expressed exclusively in astrocytes. In
both systems, 5-HT4 R-RhoA signaling changed glutamatergic
synaptic transmission: It increased the frequency of
miniature excitatory postsynaptic currents (mEPSCs) in mixed
cultures and reduced the paired-pulse-ratio (PPR) of field
excitatory postsynaptic potentials (fEPSPs) in acute slices.
Overall, our present findings demonstrate that astrocytic
5-HT4 R-Gα13 -RhoA signaling is a previously unrecognized
molecular pathway involved in the functional regulation of
excitatory synaptic circuits.},
keywords = {Astrocytes / Excitatory Postsynaptic Potentials /
Hippocampus / Receptors, Serotonin: genetics / Serotonin /
Synaptic Transmission / 5-ht Zeug (Other) / RhoA (Other) /
actin (Other) / astrocytes (Other) / neuronal excitability
(Other) / serotonin (Other)},
cin = {U Preclinical Researchers - Bonn},
ddc = {610},
cid = {I:(DE-2719)7000005},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33156956},
doi = {10.1002/glia.23933},
url = {https://pub.dzne.de/record/154261},
}