TY  - JOUR
AU  - Singh, Manvendra
AU  - Bansal, Vikas
AU  - Feschotte, Cédric
TI  - A Single-Cell RNA Expression Map of Human Coronavirus Entry Factors.
JO  - Cell reports
VL  - 32
IS  - 12
SN  - 2211-1247
CY  - [New York, NY]
PB  - Elsevier
M1  - DZNE-2021-00162
SP  - 108175
PY  - 2020
N1  - ISSN 2211-1247 not unique: **3 hits**.
AB  - To predict the tropism of human coronaviruses, we profile 28 SARS-CoV-2 and coronavirus-associated receptors and factors (SCARFs) using single-cell transcriptomics across various healthy human tissues. SCARFs include cellular factors both facilitating and restricting viral entry. Intestinal goblet cells, enterocytes, and kidney proximal tubule cells appear highly permissive to SARS-CoV-2, consistent with clinical data. Our analysis also predicts non-canonical entry paths for lung and brain infections. Spermatogonial cells and prostate endocrine cells also appear to be permissive to SARS-CoV-2 infection, suggesting male-specific vulnerabilities. Both pro- and anti-viral factors are highly expressed within the nasal epithelium, with potential age-dependent variation, predicting an important battleground for coronavirus infection. Our analysis also suggests that early embryonic and placental development are at moderate risk of infection. Lastly, SCARF expression appears broadly conserved across a subset of primate organs examined. Our study establishes a resource for investigations of coronavirus biology and pathology.
KW  - A549 Cells
KW  - Angiotensin-Converting Enzyme 2
KW  - Animals
KW  - Betacoronavirus: growth & development
KW  - COVID-19
KW  - Cell Line
KW  - Chlorocebus aethiops
KW  - Coronavirus Infections: pathology
KW  - Enterocytes: metabolism
KW  - Gene Expression Profiling
KW  - Goblet Cells: metabolism
KW  - HEK293 Cells
KW  - Humans
KW  - Kidney Tubules, Proximal: cytology
KW  - Kidney Tubules, Proximal: metabolism
KW  - Nasal Mucosa: metabolism
KW  - Nasal Mucosa: virology
KW  - Pandemics
KW  - Peptidyl-Dipeptidase A: genetics
KW  - Peptidyl-Dipeptidase A: metabolism
KW  - Pneumonia, Viral: pathology
KW  - Receptors, Virus: genetics
KW  - SARS-CoV-2
KW  - Serine Endopeptidases: genetics
KW  - Serine Endopeptidases: metabolism
KW  - Single-Cell Analysis
KW  - Vero Cells
KW  - Viral Tropism: genetics
KW  - Virus Internalization
KW  - COVID-19 (Other)
KW  - SARS-CoV-2 (Other)
KW  - coronaviruses (Other)
KW  - restriction factors (Other)
KW  - scRNA-seq (Other)
KW  - viral receptors (Other)
KW  - Receptors, Virus (NLM Chemicals)
KW  - Peptidyl-Dipeptidase A (NLM Chemicals)
KW  - ACE2 protein, human (NLM Chemicals)
KW  - Angiotensin-Converting Enzyme 2 (NLM Chemicals)
KW  - Serine Endopeptidases (NLM Chemicals)
KW  - TMPRSS2 protein, human (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:32946807
C2  - pmc:PMC7470764
DO  - DOI:10.1016/j.celrep.2020.108175
UR  - https://pub.dzne.de/record/154308
ER  -