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@ARTICLE{Singh:154308,
author = {Singh, Manvendra and Bansal, Vikas and Feschotte, Cédric},
title = {{A} {S}ingle-{C}ell {RNA} {E}xpression {M}ap of {H}uman
{C}oronavirus {E}ntry {F}actors.},
journal = {Cell reports},
volume = {32},
number = {12},
issn = {2211-1247},
address = {[New York, NY]},
publisher = {Elsevier},
reportid = {DZNE-2021-00162},
pages = {108175},
year = {2020},
note = {ISSN 2211-1247 not unique: **3 hits**.},
abstract = {To predict the tropism of human coronaviruses, we profile
28 SARS-CoV-2 and coronavirus-associated receptors and
factors (SCARFs) using single-cell transcriptomics across
various healthy human tissues. SCARFs include cellular
factors both facilitating and restricting viral entry.
Intestinal goblet cells, enterocytes, and kidney proximal
tubule cells appear highly permissive to SARS-CoV-2,
consistent with clinical data. Our analysis also predicts
non-canonical entry paths for lung and brain infections.
Spermatogonial cells and prostate endocrine cells also
appear to be permissive to SARS-CoV-2 infection, suggesting
male-specific vulnerabilities. Both pro- and anti-viral
factors are highly expressed within the nasal epithelium,
with potential age-dependent variation, predicting an
important battleground for coronavirus infection. Our
analysis also suggests that early embryonic and placental
development are at moderate risk of infection. Lastly, SCARF
expression appears broadly conserved across a subset of
primate organs examined. Our study establishes a resource
for investigations of coronavirus biology and pathology.},
keywords = {A549 Cells / Angiotensin-Converting Enzyme 2 / Animals /
Betacoronavirus: growth $\&$ development / COVID-19 / Cell
Line / Chlorocebus aethiops / Coronavirus Infections:
pathology / Enterocytes: metabolism / Gene Expression
Profiling / Goblet Cells: metabolism / HEK293 Cells / Humans
/ Kidney Tubules, Proximal: cytology / Kidney Tubules,
Proximal: metabolism / Nasal Mucosa: metabolism / Nasal
Mucosa: virology / Pandemics / Peptidyl-Dipeptidase A:
genetics / Peptidyl-Dipeptidase A: metabolism / Pneumonia,
Viral: pathology / Receptors, Virus: genetics / SARS-CoV-2 /
Serine Endopeptidases: genetics / Serine Endopeptidases:
metabolism / Single-Cell Analysis / Vero Cells / Viral
Tropism: genetics / Virus Internalization / COVID-19 (Other)
/ SARS-CoV-2 (Other) / coronaviruses (Other) / restriction
factors (Other) / scRNA-seq (Other) / viral receptors
(Other) / Receptors, Virus (NLM Chemicals) /
Peptidyl-Dipeptidase A (NLM Chemicals) / ACE2 protein, human
(NLM Chemicals) / Angiotensin-Converting Enzyme 2 (NLM
Chemicals) / Serine Endopeptidases (NLM Chemicals) / TMPRSS2
protein, human (NLM Chemicals)},
cin = {AG Bonn 1},
ddc = {610},
cid = {I:(DE-2719)1410003},
pnm = {342 - Disease Mechanisms and Model Systems (POF3-342)},
pid = {G:(DE-HGF)POF3-342},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32946807},
pmc = {pmc:PMC7470764},
doi = {10.1016/j.celrep.2020.108175},
url = {https://pub.dzne.de/record/154308},
}