%0 Journal Article
%A Schulte-Schrepping, Jonas
%A Reusch, Nico
%A Paclik, Daniela
%A Baßler, Kevin
%A Schlickeiser, Stephan
%A Zhang, Bowen
%A Krämer, Benjamin
%A Krammer, Tobias
%A Brumhard, Sophia
%A Bonaguro, Lorenzo
%A De Domenico, Elena
%A Wendisch, Daniel
%A Grasshoff, Martin
%A Kapellos, Theodore S
%A Beckstette, Michael
%A Pecht, Tal
%A Saglam, Adem
%A Dietrich, Oliver
%A Mei, Henrik E
%A Schulz, Axel R
%A Conrad, Claudia
%A Kunkel, Désirée
%A Vafadarnejad, Ehsan
%A Xu, Cheng-Jian
%A Horne, Arik
%A Herbert, Miriam
%A Drews, Anna
%A Thibeault, Charlotte
%A Pfeiffer, Moritz
%A Hippenstiel, Stefan
%A Hocke, Andreas
%A Müller-Redetzky, Holger
%A Heim, Katrin-Moira
%A Machleidt, Felix
%A Uhrig, Alexander
%A Bosquillon de Jarcy, Laure
%A Jürgens, Linda
%A Stegemann, Miriam
%A Glösenkamp, Christoph R
%A Volk, Hans-Dieter
%A Goffinet, Christine
%A Landthaler, Markus
%A Wyler, Emanuel
%A Georg, Philipp
%A Schneider, Maria
%A Dang-Heine, Chantip
%A Neuwinger, Nick
%A Kappert, Kai
%A Tauber, Rudolf
%A Corman, Victor
%A Raabe, Jan
%A Kaiser, Kim Melanie
%A Vinh, Michael To
%A Rieke, Gereon
%A Meisel, Christian
%A Ulas, Thomas
%A Becker, Matthias
%A Geffers, Robert
%A Witzenrath, Martin
%A Drosten, Christian
%A Suttorp, Norbert
%A von Kalle, Christof
%A Kurth, Florian
%A Händler, Kristian
%A Schultze, Joachim L
%A Aschenbrenner, Anna C
%A Li, Yang
%A Nattermann, Jacob
%A Sawitzki, Birgit
%A Saliba, Antoine-Emmanuel
%A Sander, Leif Erik
%A Angelov, Angel
%A Bals, Robert
%A Bartholomäus, Alexander
%A Becker, Anke
%A Bezdan, Daniela
%A Bonifacio, Ezio
%A Bork, Peer
%A Clavel, Thomas
%A Colome-Tatche, Maria
%A Diefenbach, Andreas
%A Dilthey, Alexander
%A Fischer, Nicole
%A Förstner, Konrad
%A Frick, Julia-Stefanie
%A Gagneur, Julien
%A Goesmann, Alexander
%A Hain, Torsten
%A Hummel, Michael
%A Janssen, Stefan
%A Kalinowski, Jörn
%A Kallies, René
%A Kehr, Birte
%A Keller, Andreas
%A Kim-Hellmuth, Sarah
%A Klein, Christoph
%A Kohlbacher, Oliver
%A Korbel, Jan O
%A Kurth, Ingo
%A Landthaler, Markus
%A Li, Yang
%A Ludwig, Kerstin
%A Makarewicz, Oliwia
%A Marz, Manja
%A McHardy, Alice
%A Mertes, Christian
%A Nöthen, Markus
%A Nürnberg, Peter
%A Ohler, Uwe
%A Ossowski, Stephan
%A Overmann, Jörg
%A Peter, Silke
%A Pfeffer, Klaus
%A Poetsch, Anna R
%A Pühler, Alfred
%A Rajewsky, Nikolaus
%A Ralser, Markus
%A Rieß, Olaf
%A Ripke, Stephan
%A Nunes da Rocha, Ulisses
%A Rosenstiel, Philip
%A Saliba, Antoine-Emmanuel
%A Sander, Leif Erik
%A Sawitzki, Birgit
%A Schiffer, Philipp
%A Schulte, Eva-Christina
%A Sczyrba, Alexander
%A Stegle, Oliver
%A Stoye, Jens
%A Theis, Fabian
%A Vehreschild, Janne
%A Vogel, Jörg
%A von Kleist, Max
%A Walker, Andreas
%A Walter, Jörn
%A Wieczorek, Dagmar
%A Ziebuhr, John
%T Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment.
%J Cell
%V 182
%N 6
%@ 0092-8674
%C New York, NY
%I Elsevier
%M DZNE-2021-00219
%P 1419 - 1440.e23
%D 2020
%Z ISSN 0092-8674 not unique: **3 hits**.
%X Coronavirus disease 2019 (COVID-19) is a mild to moderate respiratory tract infection, however, a subset of patients progress to severe disease and respiratory failure. The mechanism of protective immunity in mild forms and the pathogenesis of severe COVID-19 associated with increased neutrophil counts and dysregulated immune responses remain unclear. In a dual-center, two-cohort study, we combined single-cell RNA-sequencing and single-cell proteomics of whole-blood and peripheral-blood mononuclear cells to determine changes in immune cell composition and activation in mild versus severe COVID-19 (242 samples from 109 individuals) over time. HLA-DRhiCD11chi inflammatory monocytes with an interferon-stimulated gene signature were elevated in mild COVID-19. Severe COVID-19 was marked by occurrence of neutrophil precursors, as evidence of emergency myelopoiesis, dysfunctional mature neutrophils, and HLA-DRlo monocytes. Our study provides detailed insights into the systemic immune response to SARS-CoV-2 infection and reveals profound alterations in the myeloid cell compartment associated with severe COVID-19.
%K Adult
%K Aged
%K CD11 Antigens: genetics
%K CD11 Antigens: metabolism
%K COVID-19
%K Cells, Cultured
%K Coronavirus Infections: blood
%K Coronavirus Infections: immunology
%K Coronavirus Infections: pathology
%K Female
%K HLA-DR Antigens: genetics
%K HLA-DR Antigens: metabolism
%K Humans
%K Male
%K Middle Aged
%K Myeloid Cells: cytology
%K Myeloid Cells: immunology
%K Myelopoiesis
%K Pandemics
%K Pneumonia, Viral: blood
%K Pneumonia, Viral: immunology
%K Pneumonia, Viral: pathology
%K Proteome: genetics
%K Proteome: metabolism
%K Proteomics
%K Single-Cell Analysis
%K COVID-19 (Other)
%K SARS-CoV-2 (Other)
%K dysfunctional neutrophils (Other)
%K emergency myelopoiesis (Other)
%K immune profiling (Other)
%K mass cytometry (Other)
%K monocytes (Other)
%K neutrophils (Other)
%K scRNA-seq (Other)
%K CD11 Antigens (NLM Chemicals)
%K HLA-DR Antigens (NLM Chemicals)
%K Proteome (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:32810438
%2 pmc:PMC7405822
%R 10.1016/j.cell.2020.08.001
%U https://pub.dzne.de/record/154366