TY  - JOUR
AU  - Schulte-Schrepping, Jonas
AU  - Reusch, Nico
AU  - Paclik, Daniela
AU  - Baßler, Kevin
AU  - Schlickeiser, Stephan
AU  - Zhang, Bowen
AU  - Krämer, Benjamin
AU  - Krammer, Tobias
AU  - Brumhard, Sophia
AU  - Bonaguro, Lorenzo
AU  - De Domenico, Elena
AU  - Wendisch, Daniel
AU  - Grasshoff, Martin
AU  - Kapellos, Theodore S
AU  - Beckstette, Michael
AU  - Pecht, Tal
AU  - Saglam, Adem
AU  - Dietrich, Oliver
AU  - Mei, Henrik E
AU  - Schulz, Axel R
AU  - Conrad, Claudia
AU  - Kunkel, Désirée
AU  - Vafadarnejad, Ehsan
AU  - Xu, Cheng-Jian
AU  - Horne, Arik
AU  - Herbert, Miriam
AU  - Drews, Anna
AU  - Thibeault, Charlotte
AU  - Pfeiffer, Moritz
AU  - Hippenstiel, Stefan
AU  - Hocke, Andreas
AU  - Müller-Redetzky, Holger
AU  - Heim, Katrin-Moira
AU  - Machleidt, Felix
AU  - Uhrig, Alexander
AU  - Bosquillon de Jarcy, Laure
AU  - Jürgens, Linda
AU  - Stegemann, Miriam
AU  - Glösenkamp, Christoph R
AU  - Volk, Hans-Dieter
AU  - Goffinet, Christine
AU  - Landthaler, Markus
AU  - Wyler, Emanuel
AU  - Georg, Philipp
AU  - Schneider, Maria
AU  - Dang-Heine, Chantip
AU  - Neuwinger, Nick
AU  - Kappert, Kai
AU  - Tauber, Rudolf
AU  - Corman, Victor
AU  - Raabe, Jan
AU  - Kaiser, Kim Melanie
AU  - Vinh, Michael To
AU  - Rieke, Gereon
AU  - Meisel, Christian
AU  - Ulas, Thomas
AU  - Becker, Matthias
AU  - Geffers, Robert
AU  - Witzenrath, Martin
AU  - Drosten, Christian
AU  - Suttorp, Norbert
AU  - von Kalle, Christof
AU  - Kurth, Florian
AU  - Händler, Kristian
AU  - Schultze, Joachim L
AU  - Aschenbrenner, Anna C
AU  - Li, Yang
AU  - Nattermann, Jacob
AU  - Sawitzki, Birgit
AU  - Saliba, Antoine-Emmanuel
AU  - Sander, Leif Erik
AU  - Angelov, Angel
AU  - Bals, Robert
AU  - Bartholomäus, Alexander
AU  - Becker, Anke
AU  - Bezdan, Daniela
AU  - Bonifacio, Ezio
AU  - Bork, Peer
AU  - Clavel, Thomas
AU  - Colome-Tatche, Maria
AU  - Diefenbach, Andreas
AU  - Dilthey, Alexander
AU  - Fischer, Nicole
AU  - Förstner, Konrad
AU  - Frick, Julia-Stefanie
AU  - Gagneur, Julien
AU  - Goesmann, Alexander
AU  - Hain, Torsten
AU  - Hummel, Michael
AU  - Janssen, Stefan
AU  - Kalinowski, Jörn
AU  - Kallies, René
AU  - Kehr, Birte
AU  - Keller, Andreas
AU  - Kim-Hellmuth, Sarah
AU  - Klein, Christoph
AU  - Kohlbacher, Oliver
AU  - Korbel, Jan O
AU  - Kurth, Ingo
AU  - Landthaler, Markus
AU  - Li, Yang
AU  - Ludwig, Kerstin
AU  - Makarewicz, Oliwia
AU  - Marz, Manja
AU  - McHardy, Alice
AU  - Mertes, Christian
AU  - Nöthen, Markus
AU  - Nürnberg, Peter
AU  - Ohler, Uwe
AU  - Ossowski, Stephan
AU  - Overmann, Jörg
AU  - Peter, Silke
AU  - Pfeffer, Klaus
AU  - Poetsch, Anna R
AU  - Pühler, Alfred
AU  - Rajewsky, Nikolaus
AU  - Ralser, Markus
AU  - Rieß, Olaf
AU  - Ripke, Stephan
AU  - Nunes da Rocha, Ulisses
AU  - Rosenstiel, Philip
AU  - Saliba, Antoine-Emmanuel
AU  - Sander, Leif Erik
AU  - Sawitzki, Birgit
AU  - Schiffer, Philipp
AU  - Schulte, Eva-Christina
AU  - Sczyrba, Alexander
AU  - Stegle, Oliver
AU  - Stoye, Jens
AU  - Theis, Fabian
AU  - Vehreschild, Janne
AU  - Vogel, Jörg
AU  - von Kleist, Max
AU  - Walker, Andreas
AU  - Walter, Jörn
AU  - Wieczorek, Dagmar
AU  - Ziebuhr, John
TI  - Severe COVID-19 Is Marked by a Dysregulated Myeloid Cell Compartment.
JO  - Cell
VL  - 182
IS  - 6
SN  - 0092-8674
CY  - New York, NY
PB  - Elsevier
M1  - DZNE-2021-00219
SP  - 1419 - 1440.e23
PY  - 2020
N1  - ISSN 0092-8674 not unique: **3 hits**.
AB  - Coronavirus disease 2019 (COVID-19) is a mild to moderate respiratory tract infection, however, a subset of patients progress to severe disease and respiratory failure. The mechanism of protective immunity in mild forms and the pathogenesis of severe COVID-19 associated with increased neutrophil counts and dysregulated immune responses remain unclear. In a dual-center, two-cohort study, we combined single-cell RNA-sequencing and single-cell proteomics of whole-blood and peripheral-blood mononuclear cells to determine changes in immune cell composition and activation in mild versus severe COVID-19 (242 samples from 109 individuals) over time. HLA-DRhiCD11chi inflammatory monocytes with an interferon-stimulated gene signature were elevated in mild COVID-19. Severe COVID-19 was marked by occurrence of neutrophil precursors, as evidence of emergency myelopoiesis, dysfunctional mature neutrophils, and HLA-DRlo monocytes. Our study provides detailed insights into the systemic immune response to SARS-CoV-2 infection and reveals profound alterations in the myeloid cell compartment associated with severe COVID-19.
KW  - Adult
KW  - Aged
KW  - CD11 Antigens: genetics
KW  - CD11 Antigens: metabolism
KW  - COVID-19
KW  - Cells, Cultured
KW  - Coronavirus Infections: blood
KW  - Coronavirus Infections: immunology
KW  - Coronavirus Infections: pathology
KW  - Female
KW  - HLA-DR Antigens: genetics
KW  - HLA-DR Antigens: metabolism
KW  - Humans
KW  - Male
KW  - Middle Aged
KW  - Myeloid Cells: cytology
KW  - Myeloid Cells: immunology
KW  - Myelopoiesis
KW  - Pandemics
KW  - Pneumonia, Viral: blood
KW  - Pneumonia, Viral: immunology
KW  - Pneumonia, Viral: pathology
KW  - Proteome: genetics
KW  - Proteome: metabolism
KW  - Proteomics
KW  - Single-Cell Analysis
KW  - COVID-19 (Other)
KW  - SARS-CoV-2 (Other)
KW  - dysfunctional neutrophils (Other)
KW  - emergency myelopoiesis (Other)
KW  - immune profiling (Other)
KW  - mass cytometry (Other)
KW  - monocytes (Other)
KW  - neutrophils (Other)
KW  - scRNA-seq (Other)
KW  - CD11 Antigens (NLM Chemicals)
KW  - HLA-DR Antigens (NLM Chemicals)
KW  - Proteome (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:32810438
C2  - pmc:PMC7405822
DO  - DOI:10.1016/j.cell.2020.08.001
UR  - https://pub.dzne.de/record/154366
ER  -