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@ARTICLE{SchulteSchrepping:154366,
author = {Schulte-Schrepping, Jonas and Reusch, Nico and Paclik,
Daniela and Baßler, Kevin and Schlickeiser, Stephan and
Zhang, Bowen and Krämer, Benjamin and Krammer, Tobias and
Brumhard, Sophia and Bonaguro, Lorenzo and De Domenico,
Elena and Wendisch, Daniel and Grasshoff, Martin and
Kapellos, Theodore S and Beckstette, Michael and Pecht, Tal
and Saglam, Adem and Dietrich, Oliver and Mei, Henrik E and
Schulz, Axel R and Conrad, Claudia and Kunkel, Désirée and
Vafadarnejad, Ehsan and Xu, Cheng-Jian and Horne, Arik and
Herbert, Miriam and Drews, Anna and Thibeault, Charlotte and
Pfeiffer, Moritz and Hippenstiel, Stefan and Hocke, Andreas
and Müller-Redetzky, Holger and Heim, Katrin-Moira and
Machleidt, Felix and Uhrig, Alexander and Bosquillon de
Jarcy, Laure and Jürgens, Linda and Stegemann, Miriam and
Glösenkamp, Christoph R and Volk, Hans-Dieter and Goffinet,
Christine and Landthaler, Markus and Wyler, Emanuel and
Georg, Philipp and Schneider, Maria and Dang-Heine, Chantip
and Neuwinger, Nick and Kappert, Kai and Tauber, Rudolf and
Corman, Victor and Raabe, Jan and Kaiser, Kim Melanie and
Vinh, Michael To and Rieke, Gereon and Meisel, Christian and
Ulas, Thomas and Becker, Matthias and Geffers, Robert and
Witzenrath, Martin and Drosten, Christian and Suttorp,
Norbert and von Kalle, Christof and Kurth, Florian and
Händler, Kristian and Schultze, Joachim L and
Aschenbrenner, Anna C and Li, Yang and Nattermann, Jacob and
Sawitzki, Birgit and Saliba, Antoine-Emmanuel and Sander,
Leif Erik and Angelov, Angel and Bals, Robert and
Bartholomäus, Alexander and Becker, Anke and Bezdan,
Daniela and Bonifacio, Ezio and Bork, Peer and Clavel,
Thomas and Colome-Tatche, Maria and Diefenbach, Andreas and
Dilthey, Alexander and Fischer, Nicole and Förstner, Konrad
and Frick, Julia-Stefanie and Gagneur, Julien and Goesmann,
Alexander and Hain, Torsten and Hummel, Michael and Janssen,
Stefan and Kalinowski, Jörn and Kallies, René and Kehr,
Birte and Keller, Andreas and Kim-Hellmuth, Sarah and Klein,
Christoph and Kohlbacher, Oliver and Korbel, Jan O and
Kurth, Ingo and Landthaler, Markus and Li, Yang and Ludwig,
Kerstin and Makarewicz, Oliwia and Marz, Manja and McHardy,
Alice and Mertes, Christian and Nöthen, Markus and
Nürnberg, Peter and Ohler, Uwe and Ossowski, Stephan and
Overmann, Jörg and Peter, Silke and Pfeffer, Klaus and
Poetsch, Anna R and Pühler, Alfred and Rajewsky, Nikolaus
and Ralser, Markus and Rieß, Olaf and Ripke, Stephan and
Nunes da Rocha, Ulisses and Rosenstiel, Philip and Saliba,
Antoine-Emmanuel and Sander, Leif Erik and Sawitzki, Birgit
and Schiffer, Philipp and Schulte, Eva-Christina and
Sczyrba, Alexander and Stegle, Oliver and Stoye, Jens and
Theis, Fabian and Vehreschild, Janne and Vogel, Jörg and
von Kleist, Max and Walker, Andreas and Walter, Jörn and
Wieczorek, Dagmar and Ziebuhr, John},
collaboration = {Initiative, Deutsche COVID-19 OMICS},
title = {{S}evere {COVID}-19 {I}s {M}arked by a {D}ysregulated
{M}yeloid {C}ell {C}ompartment.},
journal = {Cell},
volume = {182},
number = {6},
issn = {0092-8674},
address = {New York, NY},
publisher = {Elsevier},
reportid = {DZNE-2021-00219},
pages = {1419 - 1440.e23},
year = {2020},
note = {ISSN 0092-8674 not unique: **3 hits**.},
abstract = {Coronavirus disease 2019 (COVID-19) is a mild to moderate
respiratory tract infection, however, a subset of patients
progress to severe disease and respiratory failure. The
mechanism of protective immunity in mild forms and the
pathogenesis of severe COVID-19 associated with increased
neutrophil counts and dysregulated immune responses remain
unclear. In a dual-center, two-cohort study, we combined
single-cell RNA-sequencing and single-cell proteomics of
whole-blood and peripheral-blood mononuclear cells to
determine changes in immune cell composition and activation
in mild versus severe COVID-19 (242 samples from 109
individuals) over time. HLA-DRhiCD11chi inflammatory
monocytes with an interferon-stimulated gene signature were
elevated in mild COVID-19. Severe COVID-19 was marked by
occurrence of neutrophil precursors, as evidence of
emergency myelopoiesis, dysfunctional mature neutrophils,
and HLA-DRlo monocytes. Our study provides detailed insights
into the systemic immune response to SARS-CoV-2 infection
and reveals profound alterations in the myeloid cell
compartment associated with severe COVID-19.},
keywords = {Adult / Aged / CD11 Antigens: genetics / CD11 Antigens:
metabolism / COVID-19 / Cells, Cultured / Coronavirus
Infections: blood / Coronavirus Infections: immunology /
Coronavirus Infections: pathology / Female / HLA-DR
Antigens: genetics / HLA-DR Antigens: metabolism / Humans /
Male / Middle Aged / Myeloid Cells: cytology / Myeloid
Cells: immunology / Myelopoiesis / Pandemics / Pneumonia,
Viral: blood / Pneumonia, Viral: immunology / Pneumonia,
Viral: pathology / Proteome: genetics / Proteome: metabolism
/ Proteomics / Single-Cell Analysis / COVID-19 (Other) /
SARS-CoV-2 (Other) / dysfunctional neutrophils (Other) /
emergency myelopoiesis (Other) / immune profiling (Other) /
mass cytometry (Other) / monocytes (Other) / neutrophils
(Other) / scRNA-seq (Other) / CD11 Antigens (NLM Chemicals)
/ HLA-DR Antigens (NLM Chemicals) / Proteome (NLM
Chemicals)},
cin = {Schultze - PRECISE},
ddc = {610},
cid = {I:(DE-2719)1013031},
pnm = {342 - Disease Mechanisms and Model Systems (POF3-342)},
pid = {G:(DE-HGF)POF3-342},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:32810438},
pmc = {pmc:PMC7405822},
doi = {10.1016/j.cell.2020.08.001},
url = {https://pub.dzne.de/record/154366},
}