TY  - JOUR
AU  - Kaczmarczyk, Lech
AU  - Reichenbach, Nicole
AU  - Blank, Nelli
AU  - Jonson, Maria
AU  - Dittrich, Lars
AU  - Petzold, Gabor C
AU  - Jackson, Walker S
TI  - Slc1a3-2A-CreERT2 mice reveal unique features of Bergmann glia and augment a growing collection of Cre drivers and effectors in the 129S4 genetic background.
JO  - Scientific reports
VL  - 11
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DZNE-2021-00298
SP  - 5412
PY  - 2021
AB  - Genetic variation is a primary determinant of phenotypic diversity. In laboratory mice, genetic variation can be a serious experimental confounder, and thus minimized through inbreeding. However, generalizations of results obtained with inbred strains must be made with caution, especially when working with complex phenotypes and disease models. Here we compared behavioral characteristics of C57Bl/6-the strain most widely used in biomedical research-with those of 129S4. In contrast to 129S4, C57Bl/6 demonstrated high within-strain and intra-litter behavioral hyperactivity. Although high consistency would be advantageous, the majority of disease models and transgenic tools are in C57Bl/6. We recently established six Cre driver lines and two Cre effector lines in 129S4. To augment this collection, we genetically engineered a Cre line to study astrocytes in 129S4. It was validated with two Cre effector lines: calcium indicator gCaMP5g-tdTomato and RiboTag-a tool widely used to study cell type-specific translatomes. These reporters are in different genomic loci, and in both the Cre was functional and astrocyte-specific. We found that calcium signals lasted longer and had a higher amplitude in cortical compared to hippocampal astrocytes, genes linked to a single neurodegenerative disease have highly divergent expression patterns, and that ribosome proteins are non-uniformly expressed across brain regions and cell types.
KW  - Animals
KW  - Excitatory Amino Acid Transporter 1: genetics
KW  - Excitatory Amino Acid Transporter 1: metabolism
KW  - Integrases
KW  - Mice
KW  - Mice, Transgenic
KW  - Neurodegenerative Diseases: genetics
KW  - Neurodegenerative Diseases: metabolism
KW  - Neuroglia: metabolism
LB  - PUB:(DE-HGF)16
C6  - pmid:33686166
C2  - pmc:PMC7940647
DO  - DOI:10.1038/s41598-021-84887-2
UR  - https://pub.dzne.de/record/154669
ER  -