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@ARTICLE{Kaczmarczyk:154669,
      author       = {Kaczmarczyk, Lech and Reichenbach, Nicole and Blank, Nelli
                      and Jonson, Maria and Dittrich, Lars and Petzold, Gabor C
                      and Jackson, Walker S},
      title        = {{S}lc1a3-2{A}-{C}re{ERT}2 mice reveal unique features of
                      {B}ergmann glia and augment a growing collection of {C}re
                      drivers and effectors in the 129{S}4 genetic background.},
      journal      = {Scientific reports},
      volume       = {11},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DZNE-2021-00298},
      pages        = {5412},
      year         = {2021},
      abstract     = {Genetic variation is a primary determinant of phenotypic
                      diversity. In laboratory mice, genetic variation can be a
                      serious experimental confounder, and thus minimized through
                      inbreeding. However, generalizations of results obtained
                      with inbred strains must be made with caution, especially
                      when working with complex phenotypes and disease models.
                      Here we compared behavioral characteristics of C57Bl/6-the
                      strain most widely used in biomedical research-with those of
                      129S4. In contrast to 129S4, C57Bl/6 demonstrated high
                      within-strain and intra-litter behavioral hyperactivity.
                      Although high consistency would be advantageous, the
                      majority of disease models and transgenic tools are in
                      C57Bl/6. We recently established six Cre driver lines and
                      two Cre effector lines in 129S4. To augment this collection,
                      we genetically engineered a Cre line to study astrocytes in
                      129S4. It was validated with two Cre effector lines: calcium
                      indicator gCaMP5g-tdTomato and RiboTag-a tool widely used to
                      study cell type-specific translatomes. These reporters are
                      in different genomic loci, and in both the Cre was
                      functional and astrocyte-specific. We found that calcium
                      signals lasted longer and had a higher amplitude in cortical
                      compared to hippocampal astrocytes, genes linked to a single
                      neurodegenerative disease have highly divergent expression
                      patterns, and that ribosome proteins are non-uniformly
                      expressed across brain regions and cell types.},
      keywords     = {Animals / Excitatory Amino Acid Transporter 1: genetics /
                      Excitatory Amino Acid Transporter 1: metabolism / Integrases
                      / Mice / Mice, Transgenic / Neurodegenerative Diseases:
                      genetics / Neurodegenerative Diseases: metabolism /
                      Neuroglia: metabolism},
      cin          = {AG Petzold ; AG Petzold / AG Jackson},
      ddc          = {600},
      cid          = {I:(DE-2719)1013020 / I:(DE-2719)1013019},
      pnm          = {353 - Clinical and Health Care Research (POF4-353)},
      pid          = {G:(DE-HGF)POF4-353},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33686166},
      pmc          = {pmc:PMC7940647},
      doi          = {10.1038/s41598-021-84887-2},
      url          = {https://pub.dzne.de/record/154669},
}