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@ARTICLE{PrezSisqus:154788,
author = {Pérez-Sisqués, Leticia and Sancho-Balsells, Anna and
Solana-Balaguer, Júlia and Campoy-Campos, Genís and
Vives-Isern, Marcel and Soler-Palazón, Ferran and
Anglada-Huguet, Marta and López-Toledano, Miguel-Ángel and
Mandelkow, Eva-Maria and Alberch, Jordi and Giralt, Albert
and Malagelada, Cristina},
title = {{RTP}801/{REDD}1 contributes to neuroinflammation severity
and memory impairments in {A}lzheimer’s disease},
journal = {Cell death $\&$ disease},
volume = {12},
number = {6},
issn = {2041-4889},
address = {London [u.a.]},
publisher = {Nature Publishing Group},
reportid = {DZNE-2021-00368},
pages = {616},
year = {2021},
abstract = {RTP801/REDD1 is a stress-regulated protein whose
upregulation is necessary and sufficient to trigger neuronal
death. Its downregulation in Parkinson's and Huntington's
disease models ameliorates the pathological phenotypes. In
the context of Alzheimer's disease (AD), the coding gene for
RTP801, DDIT4, is responsive to Aβ and modulates its
cytotoxicity in vitro. Also, RTP801 mRNA levels are
increased in AD patients' lymphocytes. However, the
involvement of RTP801 in the pathophysiology of AD has not
been yet tested. Here, we demonstrate that RTP801 levels are
increased in postmortem hippocampal samples from AD
patients. Interestingly, RTP801 protein levels correlated
with both Braak and Thal stages of the disease and with GFAP
expression. RTP801 levels are also upregulated in
hippocampal synaptosomal fractions obtained from murine
5xFAD and rTg4510 mice models of the disease. A local RTP801
knockdown in the 5xFAD hippocampal neurons with
shRNA-containing AAV particles ameliorates cognitive
deficits in 7-month-old animals. Upon RTP801 silencing in
the 5xFAD mice, no major changes were detected in
hippocampal synaptic markers or spine density. Importantly,
we found an unanticipated recovery of several gliosis
hallmarks and inflammasome key proteins upon neuronal RTP801
downregulation in the 5xFAD mice. Altogether our results
suggest that RTP801 could be a potential future target for
theranostic studies since it could be a biomarker of
neuroinflammation and neurotoxicity severity of the disease
and, at the same time, a promising therapeutic target in the
treatment of AD.},
keywords = {Alzheimer Disease: complications / Alzheimer Disease:
genetics / Alzheimer Disease: pathology / Animals /
Case-Control Studies / Disease Models, Animal /
Encephalitis: etiology / Encephalitis: genetics /
Encephalitis: pathology / Female / Humans / Male / Memory
Disorders: etiology / Memory Disorders: genetics / Memory
Disorders: pathology / Mice / Mice, Transgenic /
Neuroimmunomodulation: genetics / Neurotoxicity Syndromes:
etiology / Neurotoxicity Syndromes: genetics / Neurotoxicity
Syndromes: pathology / Severity of Illness Index /
Transcription Factors: physiology},
cin = {AG Mandelkow 2},
ddc = {570},
cid = {I:(DE-2719)1013015},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34131105},
pubmed = {34131105},
pmc = {pmc:PMC8206344},
doi = {10.1038/s41419-021-03899-y},
url = {https://pub.dzne.de/record/154788},
}