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@ARTICLE{Zhang:155577,
author = {Zhang, Zhengrong and Zheng, You and Niu, Zubiao and Zhang,
Bo and Wang, Chenxi and Yao, Xiaohong and Peng, Haoran and
Franca, Del Nonno and Wang, Yunyun and Zhu, Yichao and Su,
Yan and Tang, Meng and Jiang, Xiaoyi and Ren, He and He,
Meifang and Wang, Yuqi and Gao, Lihua and Zhao, Ping and
Shi, Hanping and Chen, Zhaolie and Wang, Xiaoning and
Piacentini, Mauro and Bian, Xiuwu and Melino, Gerry and Liu,
Liang and Huang, Hongyan and Sun, Qiang},
title = {{SARS}-{C}o{V}-2 spike protein dictates syncytium-mediated
lymphocyte elimination.},
journal = {Cell death and differentiation},
volume = {28},
number = {9},
issn = {1476-5403},
address = {London},
publisher = {Macmillan},
reportid = {DZNE-2021-00755},
pages = {2765-2777},
year = {2021},
abstract = {The severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) virus is highly contagious and causes
lymphocytopenia, but the underlying mechanisms are poorly
understood. We demonstrate here that heterotypic
cell-in-cell structures with lymphocytes inside
multinucleate syncytia are prevalent in the lung tissues of
coronavirus disease 2019 (COVID-19) patients. These unique
cellular structures are a direct result of SARS-CoV-2
infection, as the expression of the SARS-CoV-2 spike
glycoprotein is sufficient to induce a rapid (~45.1 nm/s)
membrane fusion to produce syncytium, which could readily
internalize multiple lines of lymphocytes to form typical
cell-in-cell structures, remarkably leading to the death of
internalized cells. This membrane fusion is dictated by a
bi-arginine motif within the polybasic S1/S2 cleavage site,
which is frequently present in the surface glycoprotein of
most highly contagious viruses. Moreover, candidate
anti-viral drugs could efficiently inhibit spike
glycoprotein processing, membrane fusion, and cell-in-cell
formation. Together, we delineate a molecular and cellular
rationale for SARS-CoV-2 pathogenesis and identify novel
targets for COVID-19 therapy.},
keywords = {COVID-19: pathology / COVID-19: virology / Cell Line / Cell
Line, Tumor / Giant Cells: pathology / Giant Cells: virology
/ HEK293 Cells / HeLa Cells / Humans / Jurkat Cells / K562
Cells / Lymphocytes: pathology / Lymphocytes: virology /
SARS-CoV-2: metabolism / SARS-CoV-2: pathogenicity / Spike
Glycoprotein, Coronavirus: metabolism / Virus
Internalization / Virus Replication: genetics},
cin = {AG Nicotera},
ddc = {610},
cid = {I:(DE-2719)5000018},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33879858},
pmc = {pmc:PMC8056997},
doi = {10.1038/s41418-021-00782-3},
url = {https://pub.dzne.de/record/155577},
}