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@ARTICLE{Shafiq:155621,
author = {Shafiq, Mohsin and Zafar, Saima and Younas, Neelam and
Noor, Aneeqa and Puig, Berta and Altmeppen, Hermann Clemens
and Schmitz, Matthias and Matschke, Jakob and Ferrer, Isidre
and Glatzel, Markus and Zerr, Inga},
title = {{P}rion protein oligomers cause neuronal cytoskeletal
damage in rapidly progressive {A}lzheimer's disease.},
journal = {Molecular neurodegeneration},
volume = {16},
number = {1},
issn = {1750-1326},
address = {London},
publisher = {Biomed Central},
reportid = {DZNE-2021-00789},
pages = {11},
year = {2021},
abstract = {High-density oligomers of the prion protein (HDPs) have
previously been identified in brain tissues of patients with
rapidly progressive Alzheimer's disease (rpAD). The current
investigation aims at identifying interacting partners of
HDPs in the rpAD brains to unravel the pathological
involvement of HDPs in the rapid progression.HDPs from the
frontal cortex tissues of rpAD brains were isolated using
sucrose density gradient centrifugation. Proteins
interacting with HDPs were identified by
co-immunoprecipitation coupled with mass spectrometry.
Further verifications were carried out using proteomic
tools, immunoblotting, and confocal laser scanning
microscopy.We identified rpAD-specific HDP-interactors,
including the growth arrest specific 2-like 2 protein
(G2L2). Intriguingly, rpAD-specific disturbances were found
in the localization of G2L2 and its associated proteins
i.e., the end binding protein 1, α-tubulin, and
β-actin.The results show the involvement of HDPs in the
destabilization of the neuronal actin/tubulin
infrastructure. We consider this disturbance to be a
contributing factor for the rapid progression in rpAD.},
keywords = {Alzheimer Disease: metabolism / Amyloid beta-Peptides:
metabolism / Brain: metabolism / Cytoskeleton: metabolism /
Cytoskeleton: pathology / Disease Progression / Humans /
Neurons: metabolism / Prion Proteins: metabolism / Actin
(Other) / Co-immunoprecipitation (Other) / Cytoskeleton
(Other) / G2L2 (Other) / GAS (Other) / Growth arrest
specific 2 like 2 (Other) / Growth arrest specific proteins
(Other) / PrPC (Other) / Prion protein oligomers (Other) /
Rapidly progressive Alzheimer’s disease (Other) / Tubulin
(Other) / rpAD (Other)},
cin = {Ext UMG Zerr / AG Zerr / Göttingen common},
ddc = {570},
cid = {I:(DE-2719)5000037 / I:(DE-2719)1440011-1 /
I:(DE-2719)6000014},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33618749},
pmc = {pmc:PMC7898440},
doi = {10.1186/s13024-021-00422-x},
url = {https://pub.dzne.de/record/155621},
}