%0 Journal Article
%A Willroider, Marie
%A Roeber, Sigrun
%A Horn, Anja K E
%A Arzberger, Thomas
%A Scheifele, Heinrich Maximilian
%A Respondek, Gesine
%A Sabri, Osama
%A Barthel, Henryk
%A Patt, Marianne
%A Mishchenko, Olena
%A Schildan, Andreas
%A Mueller, André
%A Koglin, Norman
%A Stephens, Andrew
%A Levin, Johannes
%A Höglinger, Günter U
%A Bartenstein, Peter
%A Herms, Jochen
%A Brendel, Matthias
%A Beyer, Leonie
%T Superiority of Formalin-Fixed Paraffin-Embedded Brain Tissue for in vitro Assessment of Progressive Supranuclear Palsy Tau Pathology With [ 18 F]PI-2620.
%J Frontiers in neurology
%V 12
%@ 1664-2295
%C Lausanne
%I Frontiers Research Foundation
%M DZNE-2021-00862
%P 684523
%D 2021
%X Objectives: Autoradiography on brain tissue is used to validate binding targets of newly discovered radiotracers. The purpose of this study was to correlate quantification of autoradiography signal using the novel next-generation tau positron emission tomography (PET) radiotracer [18F]PI-2620 with immunohistochemically determined tau-protein load in both formalin-fixed paraffin-embedded (FFPE) and frozen tissue samples of patients with Alzheimer's disease (AD) and Progressive Supranuclear Palsy (PSP). Methods: We applied [18F]PI-2620 autoradiography to postmortem cortical brain samples of six patients with AD, five patients with PSP and five healthy controls, respectively. Binding intensity was compared between both tissue types and different disease entities. Autoradiography signal quantification (CWMR = cortex to white matter ratio) was correlated with the immunohistochemically assessed tau load (AT8-staining, 
%K PI-2620 (Other)
%K autoradiography (Other)
%K immunohistochemistry (Other)
%K progressive supranuclear palsy (Other)
%K tau (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:34276540
%2 pmc:PMC8282895
%R 10.3389/fneur.2021.684523
%U https://pub.dzne.de/record/155694