TY  - JOUR
AU  - Willroider, Marie
AU  - Roeber, Sigrun
AU  - Horn, Anja K E
AU  - Arzberger, Thomas
AU  - Scheifele, Heinrich Maximilian
AU  - Respondek, Gesine
AU  - Sabri, Osama
AU  - Barthel, Henryk
AU  - Patt, Marianne
AU  - Mishchenko, Olena
AU  - Schildan, Andreas
AU  - Mueller, André
AU  - Koglin, Norman
AU  - Stephens, Andrew
AU  - Levin, Johannes
AU  - Höglinger, Günter U
AU  - Bartenstein, Peter
AU  - Herms, Jochen
AU  - Brendel, Matthias
AU  - Beyer, Leonie
TI  - Superiority of Formalin-Fixed Paraffin-Embedded Brain Tissue for in vitro Assessment of Progressive Supranuclear Palsy Tau Pathology With [ 18 F]PI-2620.
JO  - Frontiers in neurology
VL  - 12
SN  - 1664-2295
CY  - Lausanne
PB  - Frontiers Research Foundation
M1  - DZNE-2021-00862
SP  - 684523
PY  - 2021
AB  - Objectives: Autoradiography on brain tissue is used to validate binding targets of newly discovered radiotracers. The purpose of this study was to correlate quantification of autoradiography signal using the novel next-generation tau positron emission tomography (PET) radiotracer [18F]PI-2620 with immunohistochemically determined tau-protein load in both formalin-fixed paraffin-embedded (FFPE) and frozen tissue samples of patients with Alzheimer's disease (AD) and Progressive Supranuclear Palsy (PSP). Methods: We applied [18F]PI-2620 autoradiography to postmortem cortical brain samples of six patients with AD, five patients with PSP and five healthy controls, respectively. Binding intensity was compared between both tissue types and different disease entities. Autoradiography signal quantification (CWMR = cortex to white matter ratio) was correlated with the immunohistochemically assessed tau load (AT8-staining, 
KW  - PI-2620 (Other)
KW  - autoradiography (Other)
KW  - immunohistochemistry (Other)
KW  - progressive supranuclear palsy (Other)
KW  - tau (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:34276540
C2  - pmc:PMC8282895
DO  - DOI:10.3389/fneur.2021.684523
UR  - https://pub.dzne.de/record/155694
ER  -