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@ARTICLE{Willroider:155694,
      author       = {Willroider, Marie and Roeber, Sigrun and Horn, Anja K E and
                      Arzberger, Thomas and Scheifele, Heinrich Maximilian and
                      Respondek, Gesine and Sabri, Osama and Barthel, Henryk and
                      Patt, Marianne and Mishchenko, Olena and Schildan, Andreas
                      and Mueller, André and Koglin, Norman and Stephens, Andrew
                      and Levin, Johannes and Höglinger, Günter U and
                      Bartenstein, Peter and Herms, Jochen and Brendel, Matthias
                      and Beyer, Leonie},
      title        = {{S}uperiority of {F}ormalin-{F}ixed {P}araffin-{E}mbedded
                      {B}rain {T}issue for in vitro {A}ssessment of {P}rogressive
                      {S}upranuclear {P}alsy {T}au {P}athology {W}ith [ 18
                      {F}]{PI}-2620.},
      journal      = {Frontiers in neurology},
      volume       = {12},
      issn         = {1664-2295},
      address      = {Lausanne},
      publisher    = {Frontiers Research Foundation},
      reportid     = {DZNE-2021-00862},
      pages        = {684523},
      year         = {2021},
      abstract     = {Objectives: Autoradiography on brain tissue is used to
                      validate binding targets of newly discovered radiotracers.
                      The purpose of this study was to correlate quantification of
                      autoradiography signal using the novel next-generation tau
                      positron emission tomography (PET) radiotracer [18F]PI-2620
                      with immunohistochemically determined tau-protein load in
                      both formalin-fixed paraffin-embedded (FFPE) and frozen
                      tissue samples of patients with Alzheimer's disease (AD) and
                      Progressive Supranuclear Palsy (PSP). Methods: We applied
                      [18F]PI-2620 autoradiography to postmortem cortical brain
                      samples of six patients with AD, five patients with PSP and
                      five healthy controls, respectively. Binding intensity was
                      compared between both tissue types and different disease
                      entities. Autoradiography signal quantification (CWMR =
                      cortex to white matter ratio) was correlated with the
                      immunohistochemically assessed tau load (AT8-staining,
                      $\%-area)$ for FFPE and frozen tissue samples in the
                      different disease entities. Results: In AD tissue, relative
                      cortical tracer binding was higher in frozen samples when
                      compared to FFPE samples (CWMRfrozen vs. CWMRFFPE: 2.5-fold,
                      p < 0.001), whereas the opposite was observed in PSP tissue
                      (CWMRfrozen vs. CWMRFFPE: 0.8-fold, p = 0.004). In FFPE
                      samples, [18F]PI-2620 autoradiography tracer binding and
                      immunohistochemical tau load correlated significantly for
                      both PSP (R = 0.641, p < 0.001) and AD tissue (R = 0.435, p
                      = 0.016), indicating a high agreement of relative tracer
                      binding with underlying pathology. In frozen tissue, the
                      correlation between autoradiography and immunohistochemistry
                      was only present in AD (R = 0.417, p = 0.014) but not in PSP
                      tissue (R = -0.115, p = n.s.). Conclusion: Our head-to-head
                      comparison indicates that FFPE samples show superiority over
                      frozen samples for autoradiography assessment of PSP tau
                      pathology by [18F]PI-2620. The [18F]PI-2620 autoradiography
                      signal in FFPE samples reflects AT8 positive tau in samples
                      of both PSP and AD patients.},
      keywords     = {PI-2620 (Other) / autoradiography (Other) /
                      immunohistochemistry (Other) / progressive supranuclear
                      palsy (Other) / tau (Other)},
      cin          = {Neuropathology / Brainbank / Clinical Research (Munich) /
                      AG Herms / AG Levin},
      ddc          = {610},
      cid          = {I:(DE-2719)1140013 / I:(DE-2719)1111015 /
                      I:(DE-2719)1110001 / I:(DE-2719)1111016},
      pnm          = {353 - Clinical and Health Care Research (POF4-353) / 352 -
                      Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-353 / G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34276540},
      pmc          = {pmc:PMC8282895},
      doi          = {10.3389/fneur.2021.684523},
      url          = {https://pub.dzne.de/record/155694},
}