TY  - JOUR
AU  - Ulmke, Pauline Antonie
AU  - Sakib, M Sadman
AU  - Ditte, Peter
AU  - Sokpor, Godwin
AU  - Kerimoglu, Cemil
AU  - Pham, Linh
AU  - Xie, Yuanbin
AU  - Mao, Xiaoyi
AU  - Rosenbusch, Joachim
AU  - Teichmann, Ulrike
AU  - Nguyen, Huu Phuc
AU  - Fischer, Andre
AU  - Eichele, Gregor
AU  - Staiger, Jochen F
AU  - Tuoc, Tran
TI  - Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex.
JO  - Stem cell reports
VL  - 16
IS  - 4
SN  - 2213-6711
CY  - [New York, NY]
PB  - Elsevier
M1  - DZNE-2021-01209
SP  - 968 - 984
PY  - 2021
AB  - Intermediate progenitor cells (IPCs) are neocortical neuronal precursors. Although IPCs play crucial roles in corticogenesis, their molecular features remain largely unknown. In this study, we aimed to characterize the molecular profile of IPCs. We isolated TBR2-positive (+) IPCs and TBR2-negative (-) cell populations in the developing mouse cortex. Comparative genome-wide gene expression analysis of TBR2+ IPCs versus TBR2- cells revealed differences in key factors involved in chromatid segregation, cell-cycle regulation, transcriptional regulation, and cell signaling. Notably, mutation of many IPC genes in human has led to intellectual disability and caused a wide range of cortical malformations, including microcephaly and agenesis of corpus callosum. Loss-of-function experiments in cortex-specific mutants of Esco2, one of the novel IPC genes, demonstrate its critical role in IPC maintenance, and substantiate the identification of a central genetic determinant of IPC biogenesis. Our data provide novel molecular characteristics of IPCs in the developing mouse cortex.
KW  - Acetyltransferases: genetics
KW  - Acetyltransferases: metabolism
KW  - Animals
KW  - Apoptosis: genetics
KW  - Cerebral Cortex: cytology
KW  - Cerebral Cortex: embryology
KW  - Chromatids: metabolism
KW  - Chromosome Segregation: genetics
KW  - Gene Expression Profiling
KW  - Gene Expression Regulation
KW  - Humans
KW  - Mice
KW  - Mitosis: genetics
KW  - Mutation: genetics
KW  - Neural Stem Cells: cytology
KW  - Neural Stem Cells: metabolism
KW  - Neurodevelopmental Disorders: genetics
KW  - Neurodevelopmental Disorders: pathology
KW  - Signal Transduction
KW  - ESCO2 (Other)
KW  - TBR2 (Other)
KW  - apoptosis (Other)
KW  - cell-cycle regulation (Other)
KW  - chromosome segregation (Other)
KW  - cortical development (Other)
KW  - cortical malformation (Other)
KW  - intermediate progenitor cells (Other)
KW  - signaling pathways (Other)
KW  - transcription factors (Other)
KW  - transcriptome (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:33798452
C2  - pmc:PMC8072132
DO  - DOI:10.1016/j.stemcr.2021.03.008
UR  - https://pub.dzne.de/record/157752
ER  -