TY - JOUR AU - Safaiyani, Shima AU - Besson-Girard, Simon AU - Kaya, Tuğberk AU - Cantuti-Castelvetri, Ludovico AU - Liu, Lu AU - Ji, Hao AU - Schifferer, Martina AU - Gouna, Garyfallia AU - Usifo, Fumere AU - Kannaiyan, Nirmal AU - Fitzner, Dirk AU - Xiang, Xianyuan AU - Rossner, Moritz J AU - Brendel, Matthias AU - Gökce, Ozgun AU - Simons, Mikael TI - White matter aging drives microglial diversity. JO - Neuron VL - 109 IS - 7 SN - 0896-6273 CY - New York, NY PB - Elsevier M1 - DZNE-2021-01220 SP - 1100 - 1117.e10 PY - 2021 AB - Aging results in gray and white matter degeneration, but the specific microglial responses are unknown. Using single-cell RNA sequencing from white and gray matter separately, we identified white matter-associated microglia (WAMs), which share parts of the disease-associated microglia (DAM) gene signature and are characterized by activation of genes implicated in phagocytic activity and lipid metabolism. WAMs depend on triggering receptor expressed on myeloid cells 2 (TREM2) signaling and are aging dependent. In the aged brain, WAMs form independent of apolipoprotein E (APOE), in contrast to mouse models of Alzheimer's disease, in which microglia with the WAM gene signature are generated prematurely and in an APOE-dependent pathway similar to DAMs. Within the white matter, microglia frequently cluster in nodules, where they are engaged in clearing degenerated myelin. Thus, WAMs may represent a potentially protective response required to clear degenerated myelin accumulating during white matter aging and disease. KW - Aging: physiology KW - Alzheimer Disease: genetics KW - Animals KW - Apolipoproteins E: genetics KW - Demyelinating Diseases: pathology KW - Gene Expression Regulation KW - Gray Matter: cytology KW - Gray Matter: growth & development KW - Immunohistochemistry KW - Membrane Glycoproteins: biosynthesis KW - Membrane Glycoproteins: genetics KW - Mice KW - Mice, Inbred C57BL KW - Mice, Knockout KW - Microglia: physiology KW - Microglia: ultrastructure KW - Myelin Sheath: metabolism KW - Receptors, Immunologic: biosynthesis KW - Receptors, Immunologic: genetics KW - Sequence Analysis, RNA KW - Signal Transduction: physiology KW - Single-Cell Analysis KW - White Matter: cytology KW - White Matter: growth & development KW - ApoE (Other) KW - Trem2 (Other) KW - microglia, aging (Other) KW - myelin (Other) KW - white matter (Other) KW - Apolipoproteins E (NLM Chemicals) KW - Membrane Glycoproteins (NLM Chemicals) KW - Receptors, Immunologic (NLM Chemicals) KW - Trem2 protein, mouse (NLM Chemicals) LB - PUB:(DE-HGF)16 C6 - pmid:33606969 DO - DOI:10.1016/j.neuron.2021.01.027 UR - https://pub.dzne.de/record/157763 ER -