000157808 001__ 157808
000157808 005__ 20230915092357.0
000157808 0247_ $$2doi$$a10.3389/fcell.2021.645704
000157808 0247_ $$2pmid$$apmid:33996806
000157808 0247_ $$2pmc$$apmc:PMC8114082
000157808 0247_ $$2altmetric$$aaltmetric:104889326
000157808 037__ $$aDZNE-2021-01265
000157808 041__ $$aEnglish
000157808 082__ $$a570
000157808 1001_ $$0P:(DE-2719)2810390$$aVölkner, Manuela$$b0$$eFirst author
000157808 245__ $$aMouse Retinal Organoid Growth and Maintenance in Longer-Term Culture.
000157808 260__ $$aLausanne$$bFrontiers Media$$c2021
000157808 3367_ $$2DRIVER$$aarticle
000157808 3367_ $$2DataCite$$aOutput Types/Journal article
000157808 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1655724593_9506
000157808 3367_ $$2BibTeX$$aARTICLE
000157808 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000157808 3367_ $$00$$2EndNote$$aJournal Article
000157808 500__ $$aCC BY
000157808 520__ $$aUsing retinal organoid systems, organ-like 3D tissues, relies implicitly on their robustness. However, essential key parameters, particularly retinal growth and longer-term culture, are still insufficiently defined. Here, we hypothesize that a previously optimized protocol for high yield of evenly-sized mouse retinal organoids with low variability facilitates assessment of such parameters. We demonstrate that these organoids reliably complete retinogenesis, and can be maintained at least up to 60 days in culture. During this time, the organoids continue to mature on a molecular and (ultra)structural level: They develop photoreceptor outer segments and synapses, transiently maintain its cell composition for about 5-10 days after completing retinogenesis, and subsequently develop pathologic changes - mainly of the inner but also outer retina and reactive gliosis. To test whether this organoid system provides experimental access to the retina during and upon completion of development, we defined and stimulated organoid growth by activating sonic hedgehog signaling, which in patients and mice in vivo with a congenital defect leads to enlarged eyes. Here, a sonic hedgehog signaling activator increased retinal epithelia length in the organoid system when applied during but not after completion of development. This experimentally supports organoid maturation, stability, and experimental reproducibility in this organoid system, and provides a potential enlarged retina pathology model, as well as a protocol for producing larger organoids. Together, our study advances the understanding of retinal growth, maturation, and maintenance, and further optimizes the organoid system for future utilization.
000157808 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000157808 588__ $$aDataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
000157808 650_7 $$2Other$$adevelopment
000157808 650_7 $$2Other$$agliosis
000157808 650_7 $$2Other$$agrowth
000157808 650_7 $$2Other$$amaturation
000157808 650_7 $$2Other$$amouse
000157808 650_7 $$2Other$$aorganoid
000157808 650_7 $$2Other$$aretina
000157808 650_7 $$2Other$$asonic hedgehog
000157808 7001_ $$aKurth, Thomas$$b1
000157808 7001_ $$aSchor, Jana$$b2
000157808 7001_ $$0P:(DE-2719)2812120$$aEbner, Lynn J A$$b3
000157808 7001_ $$0P:(DE-2719)2811728$$aBardtke, Lara$$b4
000157808 7001_ $$0P:(DE-2719)9001862$$aKavak, Cagri$$b5$$udzne
000157808 7001_ $$aHackermüller, Jörg$$b6
000157808 7001_ $$0P:(DE-2719)2000041$$aKarl, Mike Oliver$$b7$$eLast author
000157808 773__ $$0PERI:(DE-600)2737824-X$$a10.3389/fcell.2021.645704$$gVol. 9, p. 645704$$p645704$$tFrontiers in cell and developmental biology$$v9$$x2296-634X$$y2021
000157808 8564_ $$uhttps://pub.dzne.de/record/157808/files/DZNE-2021-01265.pdf$$yOpenAccess
000157808 8564_ $$uhttps://pub.dzne.de/record/157808/files/DZNE-2021-01265.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000157808 909CO $$ooai:pub.dzne.de:157808$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000157808 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810390$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000157808 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2812120$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
000157808 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811728$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b4$$kDZNE
000157808 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001862$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b5$$kDZNE
000157808 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2000041$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b7$$kDZNE
000157808 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000157808 9141_ $$y2021
000157808 915__ $$0LIC:(DE-HGF)CCBYNV$$2V:(DE-HGF)$$aCreative Commons Attribution CC BY (No Version)$$bDOAJ$$d2021-01-30
000157808 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-01-30
000157808 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-01-30
000157808 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-01-30
000157808 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2021-01-30
000157808 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000157808 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2021-01-30
000157808 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bFRONT CELL DEV BIOL : 2021$$d2022-11-12
000157808 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2022-11-12
000157808 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2022-11-12
000157808 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2021-05-13T10:29:32Z
000157808 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2021-05-13T10:29:32Z
000157808 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Blind peer review$$d2021-05-13T10:29:32Z
000157808 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2022-11-12
000157808 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2022-11-12
000157808 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2022-11-12
000157808 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bFRONT CELL DEV BIOL : 2021$$d2022-11-12
000157808 9201_ $$0I:(DE-2719)1710004$$kAG Karl$$lRetinal Regeneration and Degeneration$$x0
000157808 980__ $$ajournal
000157808 980__ $$aVDB
000157808 980__ $$aUNRESTRICTED
000157808 980__ $$aI:(DE-2719)1710004
000157808 9801_ $$aFullTexts