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000162849 037__ $$aDZNE-2021-01504
000162849 041__ $$aEnglish
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000162849 1001_ $$00000-0002-1895-4772$$aNelson, Glyn$$b0
000162849 245__ $$aQUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy.
000162849 260__ $$aOxford [u.a.]$$bWiley-Blackwell$$c2021
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000162849 520__ $$aA modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated , quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist's experiments, while more than half have even failed to reproduce their own experiments. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g. DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common standards and guidelines for quality assessment and reproducibility. In April 2020, the QUality Assessment and REProducibility for instruments and images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper (1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; (2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers and observers of such; (3) outlines the current actions of the QUAREP-LiMi initiative and (4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.
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000162849 650_7 $$2Other$$aconfocal
000162849 650_7 $$2Other$$alight microscopy
000162849 650_7 $$2Other$$ametadata
000162849 650_7 $$2Other$$aquality assessment
000162849 650_7 $$2Other$$aquality control
000162849 650_7 $$2Other$$areproducibility
000162849 650_7 $$2Other$$awidefield
000162849 650_2 $$2MeSH$$aMicroscopy
000162849 650_2 $$2MeSH$$aReference Standards
000162849 650_2 $$2MeSH$$aReproducibility of Results
000162849 7001_ $$00000-0001-7471-2244$$aBoehm, Ulrike$$b1
000162849 7001_ $$aBagley, Steve$$b2
000162849 7001_ $$aBajcsy, Peter$$b3
000162849 7001_ $$aBischof, Johanna$$b4
000162849 7001_ $$aBrown, Claire M$$b5
000162849 7001_ $$aDauphin, Aurélien$$b6
000162849 7001_ $$aDobbie, Ian M$$b7
000162849 7001_ $$aEriksson, John E$$b8
000162849 7001_ $$aFaklaris, Orestis$$b9
000162849 7001_ $$aFernandez-Rodriguez, Julia$$b10
000162849 7001_ $$aFerrand, Alexia$$b11
000162849 7001_ $$aGelman, Laurent$$b12
000162849 7001_ $$aGheisari, Ali$$b13
000162849 7001_ $$aHartmann, Hella$$b14
000162849 7001_ $$aKukat, Christian$$b15
000162849 7001_ $$aLaude, Alex$$b16
000162849 7001_ $$aMitkovski, Miso$$b17
000162849 7001_ $$aMunck, Sebastian$$b18
000162849 7001_ $$aNorth, Alison J$$b19
000162849 7001_ $$aRasse, Tobias M$$b20
000162849 7001_ $$aResch-Genger, Ute$$b21
000162849 7001_ $$aSchuetz, Lucas C$$b22
000162849 7001_ $$aSeitz, Arne$$b23
000162849 7001_ $$aStrambio-De-Castillia, Caterina$$b24
000162849 7001_ $$aSwedlow, Jason R$$b25
000162849 7001_ $$0P:(DE-HGF)0$$aAlexopoulos, Ioannis$$b26
000162849 7001_ $$aAumayr, Karin$$b27
000162849 7001_ $$aAvilov, Sergiy$$b28
000162849 7001_ $$aBakker, Gert-Jan$$b29
000162849 7001_ $$aBammann, Rodrigo R$$b30
000162849 7001_ $$aBassi, Andrea$$b31
000162849 7001_ $$0P:(DE-HGF)0$$aBeckert, Hannes$$b32
000162849 7001_ $$aBeer, Sebastian$$b33
000162849 7001_ $$aBelyaev, Yury$$b34
000162849 7001_ $$aBierwagen, Jakob$$b35
000162849 7001_ $$aBirngruber, Konstantin A$$b36
000162849 7001_ $$aBosch, Manel$$b37
000162849 7001_ $$aBreitlow, Juergen$$b38
000162849 7001_ $$aCameron, Lisa A$$b39
000162849 7001_ $$aChalfoun, Joe$$b40
000162849 7001_ $$aChambers, James J$$b41
000162849 7001_ $$aChen, Chieh-Li$$b42
000162849 7001_ $$aConde-Sousa, Eduardo$$b43
000162849 7001_ $$aCorbett, Alexander D$$b44
000162849 7001_ $$aCordelieres, Fabrice P$$b45
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000162849 7001_ $$aDietzel, Ralf$$b47
000162849 7001_ $$aEismann, Frank$$b48
000162849 7001_ $$aFazeli, Elnaz$$b49
000162849 7001_ $$aFelscher, Andreas$$b50
000162849 7001_ $$0P:(DE-2719)2191568$$aFried, Hans$$b51$$udzne
000162849 7001_ $$aGaudreault, Nathalie$$b52
000162849 7001_ $$aGoh, Wah Ing$$b53
000162849 7001_ $$aGuilbert, Thomas$$b54
000162849 7001_ $$aHadleigh, Roland$$b55
000162849 7001_ $$aHemmerich, Peter$$b56
000162849 7001_ $$aHolst, Gerhard A$$b57
000162849 7001_ $$aItano, Michelle S$$b58
000162849 7001_ $$aJaffe, Claudia B$$b59
000162849 7001_ $$aJambor, Helena K$$b60
000162849 7001_ $$aJarvis, Stuart C$$b61
000162849 7001_ $$aKeppler, Antje$$b62
000162849 7001_ $$aKirchenbuechler, David$$b63
000162849 7001_ $$aKirchner, Marcel$$b64
000162849 7001_ $$aKobayashi, Norio$$b65
000162849 7001_ $$aKrens, Gabriel$$b66
000162849 7001_ $$aKunis, Susanne$$b67
000162849 7001_ $$aLacoste, Judith$$b68
000162849 7001_ $$aMarcello, Marco$$b69
000162849 7001_ $$aMartins, Gabriel G$$b70
000162849 7001_ $$aMetcalf, Daniel J$$b71
000162849 7001_ $$aMitchell, Claire A$$b72
000162849 7001_ $$aMoore, Joshua$$b73
000162849 7001_ $$aMueller, Tobias$$b74
000162849 7001_ $$aNelson, Michael S$$b75
000162849 7001_ $$aOgg, Stephen$$b76
000162849 7001_ $$aOnami, Shuichi$$b77
000162849 7001_ $$aPalmer, Alexandra L$$b78
000162849 7001_ $$aPaul-Gilloteaux, Perrine$$b79
000162849 7001_ $$aPimentel, Jaime A$$b80
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000162849 7001_ $$aSaari, Markku A$$b85
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000162849 7001_ $$aSchoonderwoert, Vincent$$b87
000162849 7001_ $$aSchroth-Diez, Britta$$b88
000162849 7001_ $$aSchwartz, Stanley$$b89
000162849 7001_ $$aShaw, Michael$$b90
000162849 7001_ $$aSpitaler, Martin$$b91
000162849 7001_ $$aStoeckl, Martin T$$b92
000162849 7001_ $$aSudar, Damir$$b93
000162849 7001_ $$aTeillon, Jeremie$$b94
000162849 7001_ $$aTerjung, Stefan$$b95
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000162849 7001_ $$aWilms, Christian D$$b97
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