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@ARTICLE{Nelson:162849,
      author       = {Nelson, Glyn and Boehm, Ulrike and Bagley, Steve and
                      Bajcsy, Peter and Bischof, Johanna and Brown, Claire M and
                      Dauphin, Aurélien and Dobbie, Ian M and Eriksson, John E
                      and Faklaris, Orestis and Fernandez-Rodriguez, Julia and
                      Ferrand, Alexia and Gelman, Laurent and Gheisari, Ali and
                      Hartmann, Hella and Kukat, Christian and Laude, Alex and
                      Mitkovski, Miso and Munck, Sebastian and North, Alison J and
                      Rasse, Tobias M and Resch-Genger, Ute and Schuetz, Lucas C
                      and Seitz, Arne and Strambio-De-Castillia, Caterina and
                      Swedlow, Jason R and Alexopoulos, Ioannis and Aumayr, Karin
                      and Avilov, Sergiy and Bakker, Gert-Jan and Bammann, Rodrigo
                      R and Bassi, Andrea and Beckert, Hannes and Beer, Sebastian
                      and Belyaev, Yury and Bierwagen, Jakob and Birngruber,
                      Konstantin A and Bosch, Manel and Breitlow, Juergen and
                      Cameron, Lisa A and Chalfoun, Joe and Chambers, James J and
                      Chen, Chieh-Li and Conde-Sousa, Eduardo and Corbett,
                      Alexander D and Cordelieres, Fabrice P and Nery, Elaine Del
                      and Dietzel, Ralf and Eismann, Frank and Fazeli, Elnaz and
                      Felscher, Andreas and Fried, Hans and Gaudreault, Nathalie
                      and Goh, Wah Ing and Guilbert, Thomas and Hadleigh, Roland
                      and Hemmerich, Peter and Holst, Gerhard A and Itano,
                      Michelle S and Jaffe, Claudia B and Jambor, Helena K and
                      Jarvis, Stuart C and Keppler, Antje and Kirchenbuechler,
                      David and Kirchner, Marcel and Kobayashi, Norio and Krens,
                      Gabriel and Kunis, Susanne and Lacoste, Judith and Marcello,
                      Marco and Martins, Gabriel G and Metcalf, Daniel J and
                      Mitchell, Claire A and Moore, Joshua and Mueller, Tobias and
                      Nelson, Michael S and Ogg, Stephen and Onami, Shuichi and
                      Palmer, Alexandra L and Paul-Gilloteaux, Perrine and
                      Pimentel, Jaime A and Plantard, Laure and Podder, Santosh
                      and Rexhepaj, Elton and Royon, Arnaud and Saari, Markku A
                      and Schapman, Damien and Schoonderwoert, Vincent and
                      Schroth-Diez, Britta and Schwartz, Stanley and Shaw, Michael
                      and Spitaler, Martin and Stoeckl, Martin T and Sudar, Damir
                      and Teillon, Jeremie and Terjung, Stefan and Thuenauer,
                      Roland and Wilms, Christian D and Wright, Graham D and
                      Nitschke, Roland},
      title        = {{QUAREP}-{L}i{M}i: {A} community-driven initiative to
                      establish guidelines for quality assessment and
                      reproducibility for instruments and images in light
                      microscopy.},
      journal      = {Journal of microscopy},
      volume       = {284},
      number       = {1},
      issn         = {1365-2818},
      address      = {Oxford [u.a.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {DZNE-2021-01504},
      pages        = {56 - 73},
      year         = {2021},
      abstract     = {A modern day light microscope has evolved from a tool
                      devoted to making primarily empirical observations to what
                      is now a sophisticated , quantitative device that is an
                      integral part of both physical and life science research.
                      Nowadays, microscopes are found in nearly every experimental
                      laboratory. However, despite their prevalent use in
                      capturing and quantifying scientific phenomena, neither a
                      thorough understanding of the principles underlying
                      quantitative imaging techniques nor appropriate knowledge of
                      how to calibrate, operate and maintain microscopes can be
                      taken for granted. This is clearly demonstrated by the
                      well-documented and widespread difficulties that are
                      routinely encountered in evaluating acquired data and
                      reproducing scientific experiments. Indeed, studies have
                      shown that more than $70\%$ of researchers have tried and
                      failed to repeat another scientist's experiments, while more
                      than half have even failed to reproduce their own
                      experiments. One factor behind the reproducibility crisis of
                      experiments published in scientific journals is the frequent
                      underreporting of imaging methods caused by a lack of
                      awareness and/or a lack of knowledge of the applied
                      technique. Whereas quality control procedures for some
                      methods used in biomedical research, such as genomics (e.g.
                      DNA sequencing, RNA-seq) or cytometry, have been introduced
                      (e.g. ENCODE), this issue has not been tackled for optical
                      microscopy instrumentation and images. Although many
                      calibration standards and protocols have been published,
                      there is a lack of awareness and agreement on common
                      standards and guidelines for quality assessment and
                      reproducibility. In April 2020, the QUality Assessment and
                      REProducibility for instruments and images in Light
                      Microscopy (QUAREP-LiMi) initiative was formed. This
                      initiative comprises imaging scientists from academia and
                      industry who share a common interest in achieving a better
                      understanding of the performance and limitations of
                      microscopes and improved quality control (QC) in light
                      microscopy. The ultimate goal of the QUAREP-LiMi initiative
                      is to establish a set of common QC standards, guidelines,
                      metadata models and tools, including detailed protocols,
                      with the ultimate aim of improving reproducible advances in
                      scientific research. This White Paper (1) summarizes the
                      major obstacles identified in the field that motivated the
                      launch of the QUAREP-LiMi initiative; (2) identifies the
                      urgent need to address these obstacles in a grassroots
                      manner, through a community of stakeholders including,
                      researchers, imaging scientists, bioimage analysts, bioimage
                      informatics developers, corporate partners, funding
                      agencies, standards organizations, scientific publishers and
                      observers of such; (3) outlines the current actions of the
                      QUAREP-LiMi initiative and (4) proposes future steps that
                      can be taken to improve the dissemination and acceptance of
                      the proposed guidelines to manage QC. To summarize, the
                      principal goal of the QUAREP-LiMi initiative is to improve
                      the overall quality and reproducibility of light microscope
                      image data by introducing broadly accepted standard
                      practices and accurately captured image data metrics.},
      keywords     = {Microscopy / Reference Standards / Reproducibility of
                      Results / confocal (Other) / light microscopy (Other) /
                      metadata (Other) / quality assessment (Other) / quality
                      control (Other) / reproducibility (Other) / widefield
                      (Other)},
      cin          = {LMF},
      ddc          = {570},
      cid          = {I:(DE-2719)1040180},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34214188},
      doi          = {10.1111/jmi.13041},
      url          = {https://pub.dzne.de/record/162849},
}