% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Zocher:162864,
      author       = {Zocher, Sara and Overall, Rupert and Berdugo-Vega, Gabriel
                      and Rund, Nicole and Karasinsky, Anne and Adusumilli, Vijay
                      S and Steinhauer, Christina and Scheibenstock, Sina and
                      Händler, Kristian and Schultze, Joachim L and Calegari,
                      Federico and Kempermann, Gerd},
      title        = {{D}e novo {DNA} methylation controls neuronal maturation
                      during adult hippocampal neurogenesis.},
      journal      = {The EMBO journal},
      volume       = {40},
      number       = {18},
      issn         = {1460-2075},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {DZNE-2021-01519},
      pages        = {e107100},
      year         = {2021},
      abstract     = {Adult neurogenesis enables the life-long addition of
                      functional neurons to the hippocampus and is regulated by
                      both cell-intrinsic molecular programs and behavioral
                      activity. De novo DNA methylation is crucial for embryonic
                      brain development, but its role during adult hippocampal
                      neurogenesis has remained unknown. Here, we show that de
                      novo DNA methylation is critical for maturation and
                      functional integration of adult-born neurons in the mouse
                      hippocampus. Bisulfite sequencing revealed that de novo DNA
                      methyltransferases target neuronal enhancers and gene bodies
                      during adult hippocampal neural stem cell differentiation,
                      to establish neuronal methylomes and facilitate
                      transcriptional up-regulation of neuronal genes. Inducible
                      deletion of both de novo DNA methyltransferases Dnmt3a and
                      Dnmt3b in adult neural stem cells did not affect
                      proliferation or fate specification, but specifically
                      impaired dendritic outgrowth and synaptogenesis of newborn
                      neurons, thereby hampering their functional maturation.
                      Consequently, abolishing de novo DNA methylation modulated
                      activation patterns in the hippocampal circuitry and caused
                      specific deficits in hippocampus-dependent learning and
                      memory. Our results demonstrate that proper establishment of
                      neuronal methylomes during adult neurogenesis is fundamental
                      for hippocampal function.},
      keywords     = {Animals / Cell Differentiation: genetics / Cells, Cultured
                      / DNA Methylation / Epigenesis, Genetic / Gene Expression
                      Regulation / Hippocampus: physiology / Mice / Neurogenesis:
                      genetics / Pyramidal Cells: cytology / Pyramidal Cells:
                      metabolism / DNA methylation (Other) / Dnmt3a (Other) /
                      adult neurogenesis (Other) / hippocampus (Other) / neuron
                      maturation (Other)},
      cin          = {AG Kempermann / AG Toda / Schultze - PRECISE / $R\&D$
                      PRECISE},
      ddc          = {570},
      cid          = {I:(DE-2719)1710001 / I:(DE-2719)1710014 /
                      I:(DE-2719)1013031 / I:(DE-2719)5000031},
      pnm          = {352 - Disease Mechanisms (POF4-352) / 354 - Disease
                      Prevention and Healthy Aging (POF4-354)},
      pid          = {G:(DE-HGF)POF4-352 / G:(DE-HGF)POF4-354},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34337766},
      pmc          = {pmc:PMC8441477},
      doi          = {10.15252/embj.2020107100},
      url          = {https://pub.dzne.de/record/162864},
}