Home > Publications Database > De novo DNA methylation controls neuronal maturation during adult hippocampal neurogenesis. > print

001     162864
005     20240301115152.0
024 7 _ |a 10.15252/embj.2020107100
|2 doi
024 7 _ |a pmid:34337766
|2 pmid
024 7 _ |a pmc:PMC8441477
|2 pmc
024 7 _ |a 0261-4189
|2 ISSN
024 7 _ |a 1460-2075
|2 ISSN
024 7 _ |a altmetric:110982832
|2 altmetric
037 _ _ |a DZNE-2021-01519
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Zocher, Sara
|0 P:(DE-2719)2810548
|b 0
|e First author
245 _ _ |a De novo DNA methylation controls neuronal maturation during adult hippocampal neurogenesis.
260 _ _ |a Hoboken, NJ [u.a.]
|c 2021
|b Wiley
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1709205778_980
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Adult neurogenesis enables the life-long addition of functional neurons to the hippocampus and is regulated by both cell-intrinsic molecular programs and behavioral activity. De novo DNA methylation is crucial for embryonic brain development, but its role during adult hippocampal neurogenesis has remained unknown. Here, we show that de novo DNA methylation is critical for maturation and functional integration of adult-born neurons in the mouse hippocampus. Bisulfite sequencing revealed that de novo DNA methyltransferases target neuronal enhancers and gene bodies during adult hippocampal neural stem cell differentiation, to establish neuronal methylomes and facilitate transcriptional up-regulation of neuronal genes. Inducible deletion of both de novo DNA methyltransferases Dnmt3a and Dnmt3b in adult neural stem cells did not affect proliferation or fate specification, but specifically impaired dendritic outgrowth and synaptogenesis of newborn neurons, thereby hampering their functional maturation. Consequently, abolishing de novo DNA methylation modulated activation patterns in the hippocampal circuitry and caused specific deficits in hippocampus-dependent learning and memory. Our results demonstrate that proper establishment of neuronal methylomes during adult neurogenesis is fundamental for hippocampal function.
536 _ _ |a 352 - Disease Mechanisms (POF4-352)
|0 G:(DE-HGF)POF4-352
|c POF4-352
|f POF IV
|x 0
536 _ _ |a 354 - Disease Prevention and Healthy Aging (POF4-354)
|0 G:(DE-HGF)POF4-354
|c POF4-354
|f POF IV
|x 1
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |a DNA methylation
|2 Other
650 _ 7 |a Dnmt3a
|2 Other
650 _ 7 |a adult neurogenesis
|2 Other
650 _ 7 |a hippocampus
|2 Other
650 _ 7 |a neuron maturation
|2 Other
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Cell Differentiation: genetics
|2 MeSH
650 _ 2 |a Cells, Cultured
|2 MeSH
650 _ 2 |a DNA Methylation
|2 MeSH
650 _ 2 |a Epigenesis, Genetic
|2 MeSH
650 _ 2 |a Gene Expression Regulation
|2 MeSH
650 _ 2 |a Hippocampus: physiology
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Neurogenesis: genetics
|2 MeSH
650 _ 2 |a Pyramidal Cells: cytology
|2 MeSH
650 _ 2 |a Pyramidal Cells: metabolism
|2 MeSH
700 1 _ |a Overall, Rupert
|0 P:(DE-2719)2812530
|b 1
700 1 _ |a Berdugo-Vega, Gabriel
|b 2
700 1 _ |a Rund, Nicole
|0 P:(DE-2719)2811674
|b 3
700 1 _ |a Karasinsky, Anne
|0 P:(DE-2719)9001964
|b 4
|u dzne
700 1 _ |a Adusumilli, Vijay S
|0 P:(DE-2719)2810887
|b 5
700 1 _ |a Steinhauer, Christina
|0 P:(DE-2719)2812839
|b 6
700 1 _ |a Scheibenstock, Sina
|0 P:(DE-2719)9001974
|b 7
|u dzne
700 1 _ |a Händler, Kristian
|0 P:(DE-2719)2812735
|b 8
700 1 _ |a Schultze, Joachim L
|0 P:(DE-2719)2811660
|b 9
700 1 _ |a Calegari, Federico
|0 0000-0002-3703-2802
|b 10
700 1 _ |a Kempermann, Gerd
|0 P:(DE-2719)2000011
|b 11
|e Last author
773 _ _ |a 10.15252/embj.2020107100
|g Vol. 40, no. 18
|0 PERI:(DE-600)1467419-1
|n 18
|p e107100
|t The EMBO journal
|v 40
|y 2021
|x 1460-2075
856 4 _ |u https://pub.dzne.de/record/162864/files/DZNE-2021-01519.pdf
|y OpenAccess
856 4 _ |u https://pub.dzne.de/record/162864/files/DZNE-2021-01519.pdf?subformat=pdfa
|x pdfa
|y OpenAccess
909 C O |o oai:pub.dzne.de:162864
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 0
|6 P:(DE-2719)2810548
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 1
|6 P:(DE-2719)2812530
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 3
|6 P:(DE-2719)2811674
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 4
|6 P:(DE-2719)9001964
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 5
|6 P:(DE-2719)2810887
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 6
|6 P:(DE-2719)2812839
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 7
|6 P:(DE-2719)9001974
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 8
|6 P:(DE-2719)2812735
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 9
|6 P:(DE-2719)2811660
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 11
|6 P:(DE-2719)2000011
913 1 _ |a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|1 G:(DE-HGF)POF4-350
|0 G:(DE-HGF)POF4-352
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Disease Mechanisms
|x 0
913 1 _ |a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|1 G:(DE-HGF)POF4-350
|0 G:(DE-HGF)POF4-354
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Disease Prevention and Healthy Aging
|x 1
914 1 _ |y 2021
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2022-11-12
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2022-11-12
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2022-11-12
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2021-01-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2022-11-12
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b EMBO J : 2021
|d 2022-11-12
915 _ _ |a IF >= 10
|0 StatID:(DE-HGF)9910
|2 StatID
|b EMBO J : 2021
|d 2022-11-12
915 _ _ |a DEAL Wiley
|0 StatID:(DE-HGF)3001
|2 StatID
|d 2021-01-30
|w ger
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2021-01-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2022-11-12
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2022-11-12
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2022-11-12
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2021-01-30
915 _ _ |a Creative Commons Attribution CC BY 4.0
|0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2022-11-12
920 1 _ |0 I:(DE-2719)1710001
|k AG Kempermann
|l Adult Neurogenesis
|x 0
920 1 _ |0 I:(DE-2719)1710014
|k AG Toda
|l Nuclear Architecture in Neural Plasticity and Aging
|x 1
920 1 _ |0 I:(DE-2719)1013031
|k Schultze - PRECISE
|l Platform for Single Cell Genomics and Epigenomics
|x 2
920 1 _ |0 I:(DE-2719)5000031
|k R&D PRECISE
|l Platform for Single Cell Genomics and Epigenomics at DZNE & University of Bonn
|x 3
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1710001
980 _ _ |a I:(DE-2719)1710014
980 _ _ |a I:(DE-2719)1013031
980 _ _ |a I:(DE-2719)5000031
980 _ _ |a UNRESTRICTED
980 1 _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21