TY - JOUR AU - Arboit, Alberto AU - Krautwald, Karla AU - Angenstein, Frank TI - The cholinergic system modulates negative BOLD responses in the prefrontal cortex once electrical perforant pathway stimulation triggers neuronal afterdischarges in the hippocampus. JO - Journal of cerebral blood flow & metabolism VL - 42 IS - 2 SN - 1559-7016 CY - London PB - Sage M1 - DZNE-2021-01576 SP - 364-380 PY - 2022 N1 - (CC BY) AB - Repeated high-frequency pulse-burst stimulations of the rat perforant pathway elicited positive BOLD responses in the right hippocampus, septum and prefrontal cortex. However, when the first stimulation period also triggered neuronal afterdischarges in the hippocampus, then a delayed negative BOLD response in the prefrontal cortex was generated. While neuronal activity and cerebral blood volume (CBV) increased in the hippocampus during the period of hippocampal neuronal afterdischarges (h-nAD), CBV decreased in the prefrontal cortex, although neuronal activity did not decrease. Only after termination of h-nAD did CBV in the prefrontal cortex increase again. Thus, h-nAD triggered neuronal activity in the prefrontal cortex that counteracted the usual neuronal activity-related functional hyperemia. This process was significantly enhanced by pilocarpine, a mACh receptor agonist, and completely blocked when pilocarpine was co-administered with scopolamine, a mACh receptor antagonist. Scopolamine did not prevent the formation of the negative BOLD response, thus mACh receptors modulate the strength of the negative BOLD response. KW - Animals KW - Cerebrovascular Circulation KW - Hippocampus: blood supply KW - Hippocampus: metabolism KW - Hyperemia: metabolism KW - Male KW - Muscarinic Agonists: pharmacology KW - Muscarinic Antagonists: pharmacology KW - Neurons: metabolism KW - Perforant Pathway: blood supply KW - Perforant Pathway: metabolism KW - Pilocarpine: pharmacology KW - Prefrontal Cortex: blood supply KW - Prefrontal Cortex: metabolism KW - Rats KW - Rats, Wistar KW - Scopolamine: pharmacology KW - Cerebral blood volume (CBV) (Other) KW - electrophysiology (Other) KW - negative BOLD response (Other) KW - pilocarpine (Other) KW - scopolamine (Other) LB - PUB:(DE-HGF)16 C2 - pmc:PMC8795231 C6 - pmid:34590894 DO - DOI:10.1177/0271678X211049820 UR - https://pub.dzne.de/record/162924 ER -