TY  - JOUR
AU  - Prisco, Luigi
AU  - Deimel, Stephan Hubertus
AU  - Yeliseyeva, Hanna
AU  - Fiala, André
AU  - Tavosanis, Gaia
TI  - The anterior paired lateral neuron normalizes odour-evoked activity in the Drosophila mushroom body calyx.
JO  - eLife
VL  - 10
SN  - 2050-084X
CY  - Cambridge
PB  - eLife Sciences Publications
M1  - DZNE-2022-00002
SP  - e74172
PY  - 2021
N1  - Grants relevant to the publication: Forschungsgruppe 2705 , Entschlüsselung eines Gehirn-Schaltkreises: Struktur, Plastizität und Verhaltensfunktion des Pilzkörpers von Drosophila
AB  - To identify and memorize discrete but similar environmental inputs, the brain needs to distinguish between subtle differences of activity patterns in defined neuronal populations. The Kenyon cells (KCs) of the Drosophila adult mushroom body (MB) respond sparsely to complex olfactory input, a property that is thought to support stimuli discrimination in the MB. To understand how this property emerges, we investigated the role of the inhibitory anterior paired lateral (APL) neuron in the input circuit of the MB, the calyx. Within the calyx, presynaptic boutons of projection neurons (PNs) form large synaptic microglomeruli (MGs) with dendrites of postsynaptic KCs. Combining electron microscopy (EM) data analysis and in vivo calcium imaging, we show that APL, via inhibitory and reciprocal synapses targeting both PN boutons and KC dendrites, normalizes odour-evoked representations in MGs of the calyx. APL response scales with the PN input strength and is regionalized around PN input distribution. Our data indicate that the formation of a sparse code by the KCs requires APL-driven normalization of their MG postsynaptic responses. This work provides experimental insights on how inhibition shapes sensory information representation in a higher brain centre, thereby supporting stimuli discrimination and allowing for efficient associative memory formation.
KW  - Animals
KW  - Calcium: analysis
KW  - Drosophila melanogaster: physiology
KW  - Female
KW  - Male
KW  - Microscopy, Confocal
KW  - Microscopy, Electron
KW  - Mushroom Bodies: physiology
KW  - Mushroom Bodies: ultrastructure
KW  - Neurons: physiology
KW  - Neurons: ultrastructure
KW  - Presynaptic Terminals
KW  - Smell: physiology
KW  - APL (Other)
KW  - D. melanogaster (Other)
KW  - inhibition (Other)
KW  - microglomerulus (Other)
KW  - mushroom body (Other)
KW  - neuroscience (Other)
KW  - pattern separation (Other)
KW  - sparse coding (Other)
KW  - Calcium (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:34964714
C2  - pmc:PMC8741211
DO  - DOI:10.7554/eLife.74172
UR  - https://pub.dzne.de/record/163151
ER  -