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@ARTICLE{Gilley:163536,
author = {Gilley, Jonathan and Jackson, Oscar and Pipis, Menelaos and
Estiar, Mehrdad A and Al-Chalabi, Ammar and Danzi, Matt C
and van Eijk, Kristel R and Goutman, Stephen A and Harms,
Matthew B and Houlden, Henry and Iacoangeli, Alfredo and
Kaye, Julia and Lima, Leandro and Ravits, John and Rouleau,
Guy A and Schüle, Rebecca and Xu, Jishu and Züchner,
Stephan and Cooper-Knock, Johnathan and Gan-Or, Ziv and
Reilly, Mary M and Coleman, Michael P},
collaboration = {Genomics, Queen Square},
title = {{E}nrichment of {SARM}1 alleles encoding variants with
constitutively hyperactive {NAD}ase in patients with {ALS}
and other motor nerve disorders.},
journal = {eLife},
volume = {10},
issn = {2050-084X},
address = {Cambridge},
publisher = {eLife Sciences Publications},
reportid = {DZNE-2022-00296},
pages = {e70905},
year = {2021},
abstract = {SARM1, a protein with critical NADase activity, is a
central executioner in a conserved programme of axon
degeneration. We report seven rare missense or in-frame
microdeletion human SARM1 variant alleles in patients with
amyotrophic lateral sclerosis (ALS) or other motor nerve
disorders that alter the SARM1 auto-inhibitory ARM domain
and constitutively hyperactivate SARM1 NADase activity. The
constitutive NADase activity of these seven variants is
similar to that of SARM1 lacking the entire ARM domain and
greatly exceeds the activity of wild-type SARM1, even in the
presence of nicotinamide mononucleotide (NMN), its
physiological activator. This rise in constitutive activity
alone is enough to promote neuronal degeneration in response
to otherwise non-harmful, mild stress. Importantly, these
strong gain-of-function alleles are completely
patient-specific in the cohorts studied and show a highly
significant association with disease at the single gene
level. These findings of disease-associated coding variants
that alter SARM1 function build on previously reported
genome-wide significant association with ALS for a
neighbouring, more common SARM1 intragenic single nucleotide
polymorphism (SNP) to support a contributory role of SARM1
in these disorders. A broad phenotypic heterogeneity and
variable age-of-onset of disease among patients with these
alleles also raises intriguing questions about the
pathogenic mechanism of hyperactive SARM1 variants.},
keywords = {Adult / Aged / Alleles / Amyotrophic Lateral Sclerosis:
genetics / Amyotrophic Lateral Sclerosis: metabolism /
Animals / Armadillo Domain Proteins / Cytoskeletal Proteins
/ Female / Humans / Male / Mice / Middle Aged / Motor Neuron
Disease: genetics / Motor Neuron Disease: metabolism / NAD+
Nucleosidase: metabolism / Nicotinamide Mononucleotide:
metabolism / ALS (Other) / NADase (Other) / SARM1 (Other) /
genetics (Other) / genomics (Other) / human (Other) /
neuroscience (Other) / risk allele (Other) / Armadillo
Domain Proteins (NLM Chemicals) / Cytoskeletal Proteins (NLM
Chemicals) / SARM1 protein, human (NLM Chemicals) /
Nicotinamide Mononucleotide (NLM Chemicals) / NAD+
Nucleosidase (NLM Chemicals)},
cin = {AG Maetzler},
ddc = {600},
cid = {I:(DE-2719)5000024},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34796871},
pmc = {pmc:PMC8735862},
doi = {10.7554/eLife.70905},
url = {https://pub.dzne.de/record/163536},
}