TY  - JOUR
AU  - Stürner, Tomke
AU  - Ferreira Castro, André
AU  - Philipps, Maren
AU  - Cuntz, Hermann
AU  - Tavosanis, Gaia
TI  - The branching code: A model of actin-driven dendrite arborization.
JO  - Cell reports
VL  - 39
IS  - 4
SN  - 2211-1247
CY  - [New York, NY]
PB  - Elsevier
M1  - DZNE-2022-00619
SP  - 110746
PY  - 2022
AB  - The cytoskeleton is crucial for defining neuronal-type-specific dendrite morphologies. To explore how the complex interplay of actin-modulatory proteins (AMPs) can define neuronal types in vivo, we focused on the class III dendritic arborization (c3da) neuron of Drosophila larvae. Using computational modeling, we reveal that the main branches (MBs) of c3da neurons follow general models based on optimal wiring principles, while the actin-enriched short terminal branches (STBs) require an additional growth program. To clarify the cellular mechanisms that define this second step, we thus concentrated on STBs for an in-depth quantitative description of dendrite morphology and dynamics. Applying these methods systematically to mutants of six known and novel AMPs, we revealed the complementary roles of these individual AMPs in defining STB properties. Our data suggest that diverse dendrite arbors result from a combination of optimal-wiring-related growth and individualized growth programs that are neuron-type specific.
KW  - Actins: metabolism
KW  - Animals
KW  - Dendrites: metabolism
KW  - Drosophila: metabolism
KW  - Drosophila Proteins: metabolism
KW  - Neuronal Plasticity
KW  - CP: Molecular biology (Other)
KW  - CP: Neuroscience (Other)
KW  - Drosophila (Other)
KW  - actin (Other)
KW  - actin-modulatory proteins (Other)
KW  - computational modeling (Other)
KW  - dendrite (Other)
KW  - dendritic arborization neurons (Other)
KW  - morphometrics (Other)
KW  - neuron (Other)
KW  - optimal wiring (Other)
KW  - time-lapse imaging (Other)
KW  - Actins (NLM Chemicals)
KW  - Drosophila Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:35476974
DO  - DOI:10.1016/j.celrep.2022.110746
UR  - https://pub.dzne.de/record/163945
ER  -