TY  - JOUR
AU  - Bartos, Laura Maria
AU  - Kunte, Sebastian Thomas
AU  - Beumers, Philipp
AU  - Xiang, Xianyuan
AU  - Wind, Karin
AU  - Ziegler, Sibylle
AU  - Bartenstein, Peter
AU  - Choi, Hongyoon
AU  - Lee, Dong Soo
AU  - Haass, Christian
AU  - von Baumgarten, Louisa
AU  - Tahirovic, Sabina
AU  - Albert, Nathalie Lisa
AU  - Lindner, Simon
AU  - Brendel, Matthias
TI  - Single cell radiotracer allocation via immunomagentic sorting (scRadiotracing) to disentangle PET signals at cellular resolution.
JO  - Journal of nuclear medicine
VL  - 63
IS  - 10
SN  - 0022-3123
CY  - New York, NY
PB  - Soc.
M1  - DZNE-2022-00775
SP  - 1459-1462
PY  - 2022
AB  - With great interest, our independent groups of scientists located in Korea and Germany recognized the use of a very similar methodological approach to quantify the uptake of radioactive glucose (18F-FDG) at the cellular level. The focus of our investigations was to disentangle microglial 18F-FDG uptake. To do so, CD11b immunomagnetic cell sorting (MACS) was applied to isolate microglia cells after in vivo 18F-FDG injection, to allow simple quantification via gamma counter. Importantly, this technique reveals a snapshot of cellular glucose uptake in living mice at the time of injection since 18F-FDG is trapped by hexokinase phosphorylation without further opportunity to be metabolized. Both studies indicated high 18F-FDG uptake of single CD11b positive microglia cells and a significant increase of microglial 18F-FDG uptake when this cell type is activated in the presence of amyloid pathology. Furthermore, another study investigated noticed that MACS after tracer injection facilitated determination of high 18F-FDG uptake in myeloid cells in a range of tumor models. Here, we aim to discuss the rationale of single cell radiotracer allocation via MACS (scRadiotracing) by providing examples of promising applications of this innovative technology in neuroscience, oncology and radiochemistry.
KW  - Neurology (Other)
KW  - PET (Other)
KW  - Research Methods (Other)
KW  - allocation (Other)
KW  - radiotracer (Other)
KW  - single cell (Other)
KW  - sorting (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:35589403
DO  - DOI:10.2967/jnumed.122.264171
UR  - https://pub.dzne.de/record/164112
ER  -