% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Bartos:164112,
      author       = {Bartos, Laura Maria and Kunte, Sebastian Thomas and
                      Beumers, Philipp and Xiang, Xianyuan and Wind, Karin and
                      Ziegler, Sibylle and Bartenstein, Peter and Choi, Hongyoon
                      and Lee, Dong Soo and Haass, Christian and von Baumgarten,
                      Louisa and Tahirovic, Sabina and Albert, Nathalie Lisa and
                      Lindner, Simon and Brendel, Matthias},
      title        = {{S}ingle cell radiotracer allocation via immunomagentic
                      sorting (sc{R}adiotracing) to disentangle {PET} signals at
                      cellular resolution.},
      journal      = {Journal of nuclear medicine},
      volume       = {63},
      number       = {10},
      issn         = {0022-3123},
      address      = {New York, NY},
      publisher    = {Soc.},
      reportid     = {DZNE-2022-00775},
      pages        = {1459-1462},
      year         = {2022},
      abstract     = {With great interest, our independent groups of scientists
                      located in Korea and Germany recognized the use of a very
                      similar methodological approach to quantify the uptake of
                      radioactive glucose (18F-FDG) at the cellular level. The
                      focus of our investigations was to disentangle microglial
                      18F-FDG uptake. To do so, CD11b immunomagnetic cell sorting
                      (MACS) was applied to isolate microglia cells after in vivo
                      18F-FDG injection, to allow simple quantification via gamma
                      counter. Importantly, this technique reveals a snapshot of
                      cellular glucose uptake in living mice at the time of
                      injection since 18F-FDG is trapped by hexokinase
                      phosphorylation without further opportunity to be
                      metabolized. Both studies indicated high 18F-FDG uptake of
                      single CD11b positive microglia cells and a significant
                      increase of microglial 18F-FDG uptake when this cell type is
                      activated in the presence of amyloid pathology. Furthermore,
                      another study investigated noticed that MACS after tracer
                      injection facilitated determination of high 18F-FDG uptake
                      in myeloid cells in a range of tumor models. Here, we aim to
                      discuss the rationale of single cell radiotracer allocation
                      via MACS (scRadiotracing) by providing examples of promising
                      applications of this innovative technology in neuroscience,
                      oncology and radiochemistry.},
      keywords     = {Neurology (Other) / PET (Other) / Research Methods (Other)
                      / allocation (Other) / radiotracer (Other) / single cell
                      (Other) / sorting (Other)},
      cin          = {AG Tahirovic / AG Haass},
      ddc          = {610},
      cid          = {I:(DE-2719)1140003 / I:(DE-2719)1110007},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:35589403},
      doi          = {10.2967/jnumed.122.264171},
      url          = {https://pub.dzne.de/record/164112},
}