% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Brugger:164282,
      author       = {Brugger, Manuel S and Baumgartner, Kathrin and Mauritz,
                      Sophie C F and Gerlach, Stefan C and Röder, Florian and
                      Schlosser, Christine and Fluhrer, Regina and Wixforth, Achim
                      and Westerhausen, Christoph},
      title        = {{V}ibration enhanced cell growth induced by surface
                      acoustic waves as in vitro wound-healing model.},
      journal      = {Proceedings of the National Academy of Sciences of the
                      United States of America},
      volume       = {117},
      number       = {50},
      issn         = {0027-8424},
      address      = {Washington, DC},
      publisher    = {National Acad. of Sciences},
      reportid     = {DZNE-2022-00936},
      pages        = {31603 - 31613},
      year         = {2020},
      abstract     = {We report on in vitro wound-healing and cell-growth studies
                      under the influence of radio-frequency (rf) cell stimuli.
                      These stimuli are supplied either by piezoactive surface
                      acoustic waves (SAWs) or by microelectrode-generated
                      electric fields, both at frequencies around 100 MHz.
                      Employing live-cell imaging, we studied the time- and
                      power-dependent healing of artificial wounds on a
                      piezoelectric chip for different cell lines. If the cell
                      stimulation is mediated by piezomechanical SAWs, we observe
                      a pronounced, significant maximum of the cell-growth rate at
                      a specific SAW amplitude, resulting in an increase of the
                      wound-healing speed of up to 135 ± $85\%$ as compared to an
                      internal reference. In contrast, cells being stimulated only
                      by electrical fields of the same magnitude as the ones
                      exposed to SAWs exhibit no significant effect. In this
                      study, we investigate this effect for different wavelengths,
                      amplitude modulation of the applied electrical rf signal,
                      and different wave modes. Furthermore, to obtain insight
                      into the biological response to the stimulus, we also
                      determined both the cell-proliferation rate and the cellular
                      stress levels. While the proliferation rate is significantly
                      increased for a wide power range, cell stress remains low
                      and within the normal range. Our findings demonstrate that
                      SAW-based vibrational cell stimulation bears the potential
                      for an alternative method to conventional ultrasound
                      treatment, overcoming some of its limitations.},
      keywords     = {Acoustic Stimulation: adverse effects / Acoustic
                      Stimulation: instrumentation / Acoustic Stimulation: methods
                      / Animals / Cell Line / Cell Line, Tumor / Cell Movement:
                      radiation effects / Cell Proliferation: radiation effects /
                      Combined Modality Therapy: adverse effects / Combined
                      Modality Therapy: instrumentation / Combined Modality
                      Therapy: methods / Dogs / Electrodes / Humans / Madin Darby
                      Canine Kidney Cells / Oxidative Stress: drug effects /
                      Reactive Oxygen Species / Sound: adverse effects /
                      Vibration: therapeutic use / Wound Healing: radiation
                      effects / cell growth (Other) / cell migration (Other) /
                      stimulation (Other) / surface acoustic waves (Other) /
                      vibration (Other) / Reactive Oxygen Species (NLM Chemicals)},
      cin          = {München common},
      ddc          = {500},
      cid          = {I:(DE-2719)6000016},
      pnm          = {899 - ohne Topic (POF4-899)},
      pid          = {G:(DE-HGF)POF4-899},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33257581},
      pmc          = {pmc:PMC7749343},
      doi          = {10.1073/pnas.2005203117},
      url          = {https://pub.dzne.de/record/164282},
}