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@ARTICLE{CantutiCastelvetri:164307,
author = {Cantuti-Castelvetri, Ludovico and Ojha, Ravi and Pedro,
Liliana Domingues and Djannatian, Minou and Franz, Jonas and
Kuivanen, Suvi and van der Meer, Franziska and Kallio, Katri
and Kaya, Tugberk and Anastasina, Maria and Smura, Teemu and
Levanov, Lev and Szirovicza, Leonora and Tobi, Allan and
Kallio-Kokko, Hannimari and Österlund, Pamela and Joensuu,
Merja and Meunier, Frédéric A and Butcher, Sarah J and
Winkler, Martin Sebastian and Mollenhauer, Brit and
Helenius, Ari and Gökce, Ozgun and Teesalu, Tambet and
Hepojoki, Jussi and Vapalahti, Olli and Stadelmann,
Christine and Balistreri, Giuseppe and Simons, Mikael},
title = {{N}europilin-1 facilitates {SARS}-{C}o{V}-2 cell entry and
infectivity.},
journal = {Science / Science now},
volume = {370},
number = {6518},
issn = {0036-8075},
address = {Washington, DC},
publisher = {Assoc.},
reportid = {DZNE-2022-00961},
pages = {856 - 860},
year = {2020},
note = {ISSN 1095-9203 not unique: **3 hits**.},
abstract = {The causative agent of coronavirus disease 2019 (COVID-19)
is the severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2). For many viruses, tissue tropism is determined
by the availability of virus receptors and entry cofactors
on the surface of host cells. In this study, we found that
neuropilin-1 (NRP1), known to bind furin-cleaved substrates,
significantly potentiates SARS-CoV-2 infectivity, an effect
blocked by a monoclonal blocking antibody against NRP1. A
SARS-CoV-2 mutant with an altered furin cleavage site did
not depend on NRP1 for infectivity. Pathological analysis of
olfactory epithelium obtained from human COVID-19 autopsies
revealed that SARS-CoV-2 infected NRP1-positive cells facing
the nasal cavity. Our data provide insight into SARS-CoV-2
cell infectivity and define a potential target for antiviral
intervention.},
keywords = {Angiotensin-Converting Enzyme 2 / Animals / Antibodies,
Monoclonal: immunology / Betacoronavirus: genetics /
Betacoronavirus: physiology / COVID-19 / Caco-2 Cells /
Coronavirus Infections: virology / Female / HEK293 Cells /
Host Microbial Interactions / Humans / Lung: metabolism /
Male / Metal Nanoparticles / Mice / Mice, Inbred C57BL /
Mutation / Neuropilin-1: chemistry / Neuropilin-1: genetics
/ Neuropilin-1: immunology / Neuropilin-1: metabolism /
Neuropilin-2: metabolism / Olfactory Mucosa: metabolism /
Olfactory Mucosa: virology / Pandemics / Peptide Fragments:
metabolism / Peptidyl-Dipeptidase A: genetics /
Peptidyl-Dipeptidase A: metabolism / Pneumonia, Viral:
virology / Protein Binding / Protein Domains / Respiratory
Mucosa: metabolism / SARS-CoV-2 / Serine Endopeptidases:
genetics / Serine Endopeptidases: metabolism / Spike
Glycoprotein, Coronavirus: chemistry / Spike Glycoprotein,
Coronavirus: metabolism / Virus Internalization /
Antibodies, Monoclonal (NLM Chemicals) / NRP1 protein, human
(NLM Chemicals) / Neuropilin-2 (NLM Chemicals) / Peptide
Fragments (NLM Chemicals) / Spike Glycoprotein, Coronavirus
(NLM Chemicals) / neuropilin-2, human (NLM Chemicals) /
spike protein, SARS-CoV-2 (NLM Chemicals) / Neuropilin-1
(NLM Chemicals) / Peptidyl-Dipeptidase A (NLM Chemicals) /
ACE2 protein, human (NLM Chemicals) / Ace2 protein, mouse
(NLM Chemicals) / Angiotensin-Converting Enzyme 2 (NLM
Chemicals) / Serine Endopeptidases (NLM Chemicals) / TMPRSS2
protein, human (NLM Chemicals)},
cin = {AG Simons},
ddc = {320},
cid = {I:(DE-2719)1110008},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:33082293},
pmc = {pmc:PMC7857391},
doi = {10.1126/science.abd2985},
url = {https://pub.dzne.de/record/164307},
}