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@ARTICLE{Coe:164565,
author = {Coe, David and Poobalasingam, Thanushiyan and Fu, Hongmei
and Bonacina, Fabrizia and Wang, Guosu and Morales, Valle
and Moregola, Annalisa and Mitro, Nico and Cheung, Kenneth
Cp and Ward, Eleanor J and Nadkarni, Suchita and
Aksentijevic, Dunja and Bianchi, Katiuscia and Norata,
Giuseppe Danilo and Capasso, Melania and Federica,
Marelli-Berg},
title = {{L}oss of voltage-gated hydrogen channel 1 expression
reveals heterogeneous metabolic adaptation to intracellular
acidification by {T} cells.},
journal = {JCI insight},
volume = {7},
number = {10},
issn = {2379-3708},
address = {Ann Arbor, Michigan},
publisher = {JCI Insight},
reportid = {DZNE-2022-01114},
pages = {e147814},
year = {2022},
note = {(CC BY 4.0)},
abstract = {Voltage-gated hydrogen channel 1 (Hvcn1) is a voltage-gated
proton channel, which reduces cytosol acidification and
facilitates the production of ROS. The increased expression
of this channel in some cancers has led to proposing Hvcn1
antagonists as potential therapeutics. While its role in
most leukocytes has been studied in depth, the function of
Hvcn1 in T cells remains poorly defined. We show that Hvcn1
plays a nonredundant role in protecting naive T cells from
intracellular acidification during priming. Despite sharing
overall functional impairment in vivo and in vitro,
Hvcn1-deficient CD4+ and CD8+ T cells display profound
differences during the transition from naive to primed T
cells, including in the preservation of T cell receptor
(TCR) signaling, cellular division, and death. These
selective features result, at least in part, from a
substantially different metabolic response to intracellular
acidification associated with priming. While Hvcn1-deficient
naive CD4+ T cells reprogram to rescue the glycolytic
pathway, naive CD8+ T cells, which express high levels of
this channel in the mitochondria, respond by metabolically
compensating mitochondrial dysfunction, at least in part via
AMPK activation. These observations imply heterogeneity
between adaptation of naive CD4+ and CD8+ T cells to
intracellular acidification during activation.},
keywords = {Hydrogen / Hydrogen-Ion Concentration / Lymphocyte Count /
Protons / Signal Transduction / Adaptive immunity (Other) /
Immunology (Other) / Protons (NLM Chemicals) / Hydrogen (NLM
Chemicals)},
cin = {AG Capasso},
ddc = {610},
cid = {I:(DE-2719)1013033},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pmc = {pmc:PMC9220931},
pubmed = {pmid:35472029},
doi = {10.1172/jci.insight.147814},
url = {https://pub.dzne.de/record/164565},
}