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000165227 037__ $$aDZNE-2022-01524
000165227 041__ $$aEnglish
000165227 082__ $$a610
000165227 1001_ $$aKroon, Cristina$$b0
000165227 245__ $$aBlind spots on western blots: Assessment of common problems in western blot figures and methods reporting with recommendations to improve them.
000165227 260__ $$aLawrence, KS$$bPLoS$$c2022
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000165227 520__ $$aWestern blotting is a standard laboratory method used to detect proteins and assess their expression levels. Unfortunately, poor western blot image display practices and a lack of detailed methods reporting can limit a reader's ability to evaluate or reproduce western blot results. While several groups have studied the prevalence of image manipulation or provided recommendations for improving western blotting, data on the prevalence of common publication practices are scarce. We systematically examined 551 articles published in the top 25% of journals in neurosciences (n = 151) and cell biology (n = 400) that contained western blot images, focusing on practices that may omit important information. Our data show that most published western blots are cropped and blot source data are not made available to readers in the supplement. Publishing blots with visible molecular weight markers is rare, and many blots additionally lack molecular weight labels. Western blot methods sections often lack information on the amount of protein loaded on the gel, blocking steps, and antibody labeling protocol. Important antibody identifiers like company or supplier, catalog number, or RRID were omitted frequently for primary antibodies and regularly for secondary antibodies. We present detailed descriptions and visual examples to help scientists, peer reviewers, and editors to publish more informative western blot figures and methods. Additional resources include a toolbox to help scientists produce more reproducible western blot data, teaching slides in English and Spanish, and an antibody reporting template.
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000165227 650_7 $$2NLM Chemicals$$aAntibodies
000165227 650_7 $$2NLM Chemicals$$aProteins
000165227 650_2 $$2MeSH$$aAntibodies
000165227 650_2 $$2MeSH$$aBlotting, Western
000165227 650_2 $$2MeSH$$aNeurosciences
000165227 650_2 $$2MeSH$$aProteins
000165227 7001_ $$0P:(DE-2719)9000500$$aBreuer, Larissa$$b1$$udzne
000165227 7001_ $$aJones, Lydia$$b2
000165227 7001_ $$aAn, Jeehye$$b3
000165227 7001_ $$aAkan, Ayça$$b4
000165227 7001_ $$aMohamed Ali, Elkhansa Ahmed$$b5
000165227 7001_ $$aBusch, Felix$$b6
000165227 7001_ $$aFislage, Marinus$$b7
000165227 7001_ $$aGhosh, Biswajit$$b8
000165227 7001_ $$aHellrigel-Holderbaum, Max$$b9
000165227 7001_ $$aKazezian, Vartan$$b10
000165227 7001_ $$aKoppold, Alina$$b11
000165227 7001_ $$aMoreira Restrepo, Cesar Alberto$$b12
000165227 7001_ $$aRiedel, Nico$$b13
000165227 7001_ $$aScherschinski, Lea$$b14
000165227 7001_ $$aUrrutia Gonzalez, Fernando Raúl$$b15
000165227 7001_ $$00000-0002-7490-2600$$aWeissgerber, Tracey L$$b16
000165227 773__ $$0PERI:(DE-600)2126773-X$$a10.1371/journal.pbio.3001783$$gVol. 20, no. 9, p. e3001783 -$$n9$$pe3001783$$tPLoS biology$$v20$$x1544-9173$$y2022
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