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@ARTICLE{Kroon:165227,
      author       = {Kroon, Cristina and Breuer, Larissa and Jones, Lydia and
                      An, Jeehye and Akan, Ayça and Mohamed Ali, Elkhansa Ahmed
                      and Busch, Felix and Fislage, Marinus and Ghosh, Biswajit
                      and Hellrigel-Holderbaum, Max and Kazezian, Vartan and
                      Koppold, Alina and Moreira Restrepo, Cesar Alberto and
                      Riedel, Nico and Scherschinski, Lea and Urrutia Gonzalez,
                      Fernando Raúl and Weissgerber, Tracey L},
      title        = {{B}lind spots on western blots: {A}ssessment of common
                      problems in western blot figures and methods reporting with
                      recommendations to improve them.},
      journal      = {PLoS biology},
      volume       = {20},
      number       = {9},
      issn         = {1544-9173},
      address      = {Lawrence, KS},
      publisher    = {PLoS},
      reportid     = {DZNE-2022-01524},
      pages        = {e3001783},
      year         = {2022},
      note         = {CC BY: https://creativecommons.org/licenses/by/4.0/},
      abstract     = {Western blotting is a standard laboratory method used to
                      detect proteins and assess their expression levels.
                      Unfortunately, poor western blot image display practices and
                      a lack of detailed methods reporting can limit a reader's
                      ability to evaluate or reproduce western blot results. While
                      several groups have studied the prevalence of image
                      manipulation or provided recommendations for improving
                      western blotting, data on the prevalence of common
                      publication practices are scarce. We systematically examined
                      551 articles published in the top $25\%$ of journals in
                      neurosciences (n = 151) and cell biology (n = 400) that
                      contained western blot images, focusing on practices that
                      may omit important information. Our data show that most
                      published western blots are cropped and blot source data are
                      not made available to readers in the supplement. Publishing
                      blots with visible molecular weight markers is rare, and
                      many blots additionally lack molecular weight labels.
                      Western blot methods sections often lack information on the
                      amount of protein loaded on the gel, blocking steps, and
                      antibody labeling protocol. Important antibody identifiers
                      like company or supplier, catalog number, or RRID were
                      omitted frequently for primary antibodies and regularly for
                      secondary antibodies. We present detailed descriptions and
                      visual examples to help scientists, peer reviewers, and
                      editors to publish more informative western blot figures and
                      methods. Additional resources include a toolbox to help
                      scientists produce more reproducible western blot data,
                      teaching slides in English and Spanish, and an antibody
                      reporting template.},
      keywords     = {Antibodies / Blotting, Western / Neurosciences / Proteins /
                      Antibodies (NLM Chemicals) / Proteins (NLM Chemicals)},
      cin          = {AG Dean},
      ddc          = {610},
      cid          = {I:(DE-2719)1813003},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36095010},
      pmc          = {pmc:PMC9518894},
      doi          = {10.1371/journal.pbio.3001783},
      url          = {https://pub.dzne.de/record/165227},
}