TY  - JOUR
AU  - Shahriyari, Mina
AU  - Islam, Rezaul
AU  - Sakib, Sadman M
AU  - Rinn, Malte
AU  - Rika, Anastasia
AU  - Krüger, Dennis
AU  - Kaurani, Lalit
AU  - Gisa, Verena
AU  - Winterhoff, Mandy
AU  - Anandakumar, Harithaa
AU  - Shomroni, Orr
AU  - Schmidt, Matthias
AU  - Salinas, Gabriela
AU  - Unger, Andreas
AU  - Linke, Wolfgang A
AU  - Zschüntzsch, Jana
AU  - Schmidt, Jens
AU  - Bassel-Duby, Rhonda
AU  - Olson, Eric N
AU  - Fischer, André
AU  - Zimmermann, Wolfram-Hubertus
AU  - Tiburcy, Malte
TI  - Engineered skeletal muscle recapitulates human muscle development, regeneration and dystrophy.
JO  - Journal of cachexia, sarcopenia and muscle
VL  - 13
IS  - 6
SN  - 2190-5991
CY  - Hoboken, NJ
PB  - Wiley
M1  - DZNE-2022-01596
SP  - 3106-3121
PY  - 2022
AB  - Human pluripotent stem cell-derived muscle models show great potential for translational research. Here, we describe developmentally inspired methods for the derivation of skeletal muscle cells and their utility in skeletal muscle tissue engineering with the aim to model skeletal muscle regeneration and dystrophy in vitro.Key steps include the directed differentiation of human pluripotent stem cells to embryonic muscle progenitors followed by primary and secondary foetal myogenesis into three-dimensional muscle. To simulate Duchenne muscular dystrophy (DMD), a patient-specific induced pluripotent stem cell line was compared to a CRISPR/Cas9-edited isogenic control line.The established skeletal muscle differentiation protocol robustly and faithfully recapitulates critical steps of embryonic myogenesis in two-dimensional and three-dimensional cultures, resulting in functional human skeletal muscle organoids (SMOs) and engineered skeletal muscles (ESMs) with a regeneration-competent satellite-like cell pool. Tissue-engineered muscle exhibits organotypic maturation and function (up to 5.7 ± 0.5 mN tetanic twitch tension at 100 Hz in ESM). Contractile performance could be further enhanced by timed thyroid hormone treatment, increasing the speed of contraction (time to peak contraction) as well as relaxation (time to 50
KW  - Humans
KW  - Muscular Dystrophy, Duchenne: genetics
KW  - Muscle, Skeletal: metabolism
KW  - Muscle Development: genetics
KW  - Satellite Cells, Skeletal Muscle: metabolism
KW  - Muscle Fibers, Skeletal: metabolism
KW  - Duchenne muscular dystrophy (Other)
KW  - hypaxial dermomyotome (Other)
KW  - limb muscle (Other)
KW  - satellite cells (Other)
KW  - skeletal muscle organoid (Other)
KW  - somite (Other)
KW  - tissue engineering (Other)
LB  - PUB:(DE-HGF)16
C2  - pmc:PMC9745484
C6  - pmid:36254806
DO  - DOI:10.1002/jcsm.13094
UR  - https://pub.dzne.de/record/165318
ER  -