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@ARTICLE{PrezArvalo:165344,
      author       = {Pérez Arévalo, Andrea and Lutz, Anne-Kathrin and
                      Atanasova, Ekaterina and Böckers, Tobias},
      title        = {{T}rans-cardiac perfusion of neonatal mice and
                      immunofluorescence of the whole body as a method to study
                      nervous system development.},
      journal      = {PLOS ONE},
      volume       = {17},
      number       = {10},
      issn         = {1932-6203},
      address      = {San Francisco, California, US},
      publisher    = {PLOS},
      reportid     = {DZNE-2022-01622},
      pages        = {e0275780},
      year         = {2022},
      abstract     = {Whole animal perfusion is a well-established method that
                      has been used for the past decades in multiple research
                      fields. Particularly, it has been very important for the
                      study of the brain. The rapid and uniform fixation of tissue
                      is essential for the preservation of its integrity and the
                      study of complex structures. For small tissue pieces
                      submerging in formaldehyde solution oftentimes is sufficient
                      to get a good fixation, larger tissues or organs with a more
                      complicated structure present a greater difficulty. Here, we
                      report the precise parameters to successfully perform
                      trans-cardiac perfusion of neonatal mouse pups that allows a
                      uniform fixation of the whole body for subsequent structural
                      analysis and immunohistochemistry. In comparison to standard
                      perfusion procedures of adult mice, changes in the pump
                      velocity, the buffer volume and in the needle size lead to
                      high quality fixation of neonatal mice pups. Further, we
                      present a whole-body section staining, which results in a
                      highly specific immunofluorescence signal suited for
                      detailed analysis of multiple tissues or systems at the same
                      time. Thus, our protocol provides a reproducible and
                      reliable method for neonatal perfusion and staining that can
                      rapidly be applied in any laboratory. It allows a high
                      quality analysis of cellular structures and expression
                      profiles at early developmental stages.},
      keywords     = {Animals / Animals, Newborn / Brain / Fluorescent Antibody
                      Technique / Formaldehyde / Immunohistochemistry / Mice /
                      Perfusion: methods / Staining and Labeling / Tissue
                      Fixation: methods / Formaldehyde (NLM Chemicals)},
      cin          = {AG Böckers},
      ddc          = {610},
      cid          = {I:(DE-2719)1910002},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36227942},
      pmc          = {pmc:PMC9560478},
      doi          = {10.1371/journal.pone.0275780},
      url          = {https://pub.dzne.de/record/165344},
}