TY  - CHAP
AU  - Kaniyappan, Senthilvelrajan
AU  - Balaji, Varun
AU  - Wang, Yipeng
AU  - Mandelkow, Eckhard
TI  - Microfluidic Chamber Technology to Study Missorting and Spreading of Tau Protein in Alzheimer's Disease.
VL  - 2551
CY  - New York, NY
PB  - Springer US
M1  - DZNE-2022-01707
SN  - 978-1-0716-2596-5 (print)
T2  - Methods in Molecular Biology
SP  - 111 - 123
PY  - 2023
AB  - Tau is a microtubule-associated protein found mainly in the axons of neurons in the brain. Abnormal changes in Tau (e.g., aggregation, hyperphosphorylation) are hallmarks of Alzheimer's disease. Two processes of relocalization of Tau may be related to early states of the pathology and have received much attention: (1) the redistribution of Tau within cells (termed 'somatodendritic missorting') and (2) the release and reuptake of Tau from donor to acceptor cells (termed 'spreading'). Because of the tripartite nature of neurons (cell body, dendrites, axons), these changes can be studied by microfluidic chambers (MFCs) which allow separation and observation of Tau in neuronal compartments. In this chapter, we present some methods and research results obtained by using microfluidic devices.
KW  - Humans
KW  - tau Proteins: metabolism
KW  - Alzheimer Disease: metabolism
KW  - Microfluidics
KW  - Neurons: metabolism
KW  - Axons: metabolism
KW  - Microfluidic chambers (Other)
KW  - Missorting (Other)
KW  - Primary neurons (Other)
KW  - Spreading (Other)
KW  - Tau (Other)
KW  - tau Proteins (NLM Chemicals)
LB  - PUB:(DE-HGF)7
C6  - pmid:36310200
DO  - DOI:10.1007/978-1-0716-2597-2_9
UR  - https://pub.dzne.de/record/165567
ER  -