TY - JOUR AU - Kim, Tae-Kyung AU - Bae, Eun-Jin AU - Jung, Byung Chul AU - Choi, Minsun AU - Shin, Soo Jean AU - Park, Sung Jun AU - Kim, Jeong Tae AU - Jung, Min Kyo AU - Ulusoy, Ayse AU - Song, Mi-Young AU - Lee, Jun Sung AU - Lee, He-Jin AU - Di Monte, Donato A AU - Lee, Seung-Jae TI - Inflammation promotes synucleinopathy propagation. JO - Experimental & molecular medicine VL - 54 IS - 12 SN - 2092-6413 CY - Seoul PB - Soc. M1 - DZNE-2023-00012 SP - 2148-2161 PY - 2022 N1 - ISSN 2092-6413 not unique: **2 hits**. AB - The clinical progression of neurodegenerative diseases correlates with the spread of proteinopathy in the brain. The current understanding of the mechanism of proteinopathy spread is far from complete. Here, we propose that inflammation is fundamental to proteinopathy spread. A sequence variant of α-synuclein (V40G) was much less capable of fibril formation than wild-type α-synuclein (WT-syn) and, when mixed with WT-syn, interfered with its fibrillation. However, when V40G was injected intracerebrally into mice, it induced aggregate spreading even more effectively than WT-syn. Aggregate spreading was preceded by sustained microgliosis and inflammatory responses, which were more robust with V40G than with WT-syn. Oral administration of an anti-inflammatory agent suppressed aggregate spreading, inflammation, and behavioral deficits in mice. Furthermore, exposure of cells to inflammatory cytokines increased the cell-to-cell propagation of α-synuclein. These results suggest that the inflammatory microenvironment is the major driver of the spread of synucleinopathy in the brain. KW - Mice KW - Animals KW - alpha-Synuclein: genetics KW - alpha-Synuclein: metabolism KW - Synucleinopathies KW - Brain: metabolism KW - Neurodegenerative Diseases KW - Inflammation KW - Disease Models, Animal KW - alpha-Synuclein (NLM Chemicals) LB - PUB:(DE-HGF)16 C2 - pmc:PMC9794777 C6 - pmid:36473937 DO - DOI:10.1038/s12276-022-00895-w UR - https://pub.dzne.de/record/169133 ER -