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000169154 1001_ $$0P:(DE-2719)9000766$$aSilva-Correia, Susana$$b0$$eFirst author$$udzne
000169154 245__ $$aRole of different recombinant PrP substrates in the diagnostic accuracy of the CSF RT-QuIC assay in Creutzfeldt-Jakob disease.
000169154 260__ $$aHeidelberg$$bSpringer$$c2023
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000169154 520__ $$aThe development of the real-time quaking-induced conversion (RT-QuIC), an in vitro protein misfolding amplification assay, was an innovation in the scientific field of protein misfolding diseases. In prion diseases, these types of assays imitate the pathological conversion of the cellular prion protein (PrPC) into a protease-resistant and/or amyloid form of PrP, called PrP resistant (PrPRes). The RT-QuIC is an automatic assay system based on real-time measuring of thioflavin-T (Th-T) incorporation into amyloid fibrils using shaking for disaggregation. It has already been applied in diagnostics, drug pre-screening, and to distinguish between different prion strains. The seeded conversion efficiency and the diagnostic accuracy of the RT-QuIC assay strongly depend on the kind of recombinant PrP (rec PrP) substrate. The DNA sequences of different substrates may originate from different species, such as human, bank vole, and hamster, or from a combination of two species, e.g., hamster-sheep chimera. In routine use, either full-length (FL) or truncated substrates are applied which can accelerate the conversion reaction, e.g., to a more sensitive version of RT-QuIC assay. In the present review, we provide an overview on the different types of PrP substrates (FL and truncated forms), recapitulate the production and purification process of different rec PrP substrates, and discuss the diagnostic value of CSF RT-QuIC in human prion disease diagnostics.
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000169154 650_7 $$2NLM Chemicals$$aPrions
000169154 650_7 $$2Other$$aCreutzfeldt-Jakob disease
000169154 650_7 $$2Other$$aDiagnostics
000169154 650_7 $$2Other$$aReal-time quaking-induced conversion
000169154 650_7 $$2Other$$aRecombinant prion protein substrates
000169154 650_7 $$2NLM Chemicals$$aPrion Proteins
000169154 650_2 $$2MeSH$$aCricetinae
000169154 650_2 $$2MeSH$$aHumans
000169154 650_2 $$2MeSH$$aAnimals
000169154 650_2 $$2MeSH$$aSheep
000169154 650_2 $$2MeSH$$aCreutzfeldt-Jakob Syndrome: diagnosis
000169154 650_2 $$2MeSH$$aPrions: metabolism
000169154 650_2 $$2MeSH$$aPrion Proteins: metabolism
000169154 7001_ $$0P:(DE-2719)9000287$$aSchmitz, Matthias$$b1$$udzne
000169154 7001_ $$0P:(DE-2719)2000047$$aFischer, Andre$$b2$$udzne
000169154 7001_ $$0P:(DE-2719)2812183$$aHermann, Peter$$b3$$udzne
000169154 7001_ $$0P:(DE-2719)2000058$$aZerr, Inga$$b4$$eLast author$$udzne
000169154 773__ $$0PERI:(DE-600)1458496-7$$a10.1007/s00441-022-03715-9$$n1$$p301-306$$tCell & tissue research$$v392$$x2192-5917$$y2023
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