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@ARTICLE{Abyzov:169187,
author = {Abyzov, Anton and Mandelkow, Eckhard and Zweckstetter,
Markus and Rezaei-Ghaleh, Nasrollah},
title = {{F}ast {M}otions {D}ominate {D}ynamics of {I}ntrinsically
{D}isordered {T}au {P}rotein at {H}igh {T}emperatures.},
journal = {Chemistry - a European journal},
volume = {29},
number = {17},
issn = {0947-6539},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {DZNE-2023-00066},
pages = {e202203493},
year = {2023},
abstract = {Reorientational dynamics of intrinsically disordered
proteins (IDPs) contain multiple motions often clustered
around three motional modes: ultrafast librational motions
of amide groups, fast local backbone conformational
fluctuations and slow chain segmental motions. This dynamic
picture is mainly based on 15 N NMR relaxation studies of
IDPs at relatively low temperatures where the amide-water
proton exchange rates are sufficiently small. Less is known,
however, about the dynamics of IDPs at more physiological
temperatures. Here, we investigate protein dynamics in a
441-residue long IDP, tau protein, in the temperature range
from 0-25 °C, using 15 N NMR relaxation rates and spectral
density analysis. While at these temperatures relaxation
rates are still better described in terms of amide group
librational motions, local backbone dynamics and chain
segmental motions, the temperature-dependent trend of
spectral densities suggests that the timescales of fast
backbone conformational fluctuations and slower chain
segmental motions might become inseparable at higher
temperatures. Our data demonstrate the remarkable dynamic
plasticity of this prototypical IDP and highlight the need
for dynamic studies of IDPs at multiple temperatures.},
keywords = {Temperature / Protein Conformation / tau Proteins /
Magnetic Resonance Spectroscopy / Intrinsically Disordered
Proteins: chemistry / Amides / tau Proteins (NLM Chemicals)
/ Intrinsically disordered protein (Other) / NMR relaxation
(Other) / protein dynamics (Other) / spectral density
analysis (Other) / tau protein (Other) / Intrinsically
Disordered Proteins (NLM Chemicals) / Amides (NLM
Chemicals)},
cin = {AG Mandelkow 1 / AG Zweckstetter},
ddc = {540},
cid = {I:(DE-2719)1013014 / I:(DE-2719)1410001},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:36579699},
doi = {10.1002/chem.202203493},
url = {https://pub.dzne.de/record/169187},
}