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000169351 1001_ $$aNitta, Yohei$$b0
000169351 245__ $$aDirect evaluation of neuroaxonal degeneration with the causative genes of neurodegenerative diseases in drosophila using the automated axon quantification system, MeDUsA.
000169351 260__ $$aOxford$$bOxford Univ. Press$$c2023
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000169351 520__ $$aDrosophila is an excellent model organism for studying human neurodegenerative diseases (NDs). However, there is still almost no experimental system that could directly observe the degeneration of neurons and automatically quantify axonal degeneration. In this study, we created MeDUsA (a 'method for the quantification of degeneration using fly axons'), a standalone executable computer program based on Python that combines a pre-trained deep-learning masking tool with an axon terminal counting tool. This software automatically quantifies the number of retinal R7 axons in Drosophila from a confocal z-stack image series. Using this software, we were able to directly demonstrate that axons were degenerated by the representative causative genes of NDs for the first time in Drosophila. The fly retinal axon is an excellent experimental system that is capable of mimicking the pathology of axonal degeneration in human NDs. MeDUsA rapidly and accurately quantifies axons in Drosophila photoreceptor neurons. It enables large-scale research into axonal degeneration, including screening to identify genes or drugs that mediate axonal toxicity caused by ND proteins and diagnose the pathological significance of novel variants of human genes in axons.
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000169351 650_7 $$2NLM Chemicals$$aDrosophila Proteins
000169351 650_2 $$2MeSH$$aAnimals
000169351 650_2 $$2MeSH$$aHumans
000169351 650_2 $$2MeSH$$aDrosophila: genetics
000169351 650_2 $$2MeSH$$aDrosophila: metabolism
000169351 650_2 $$2MeSH$$aNeurodegenerative Diseases: metabolism
000169351 650_2 $$2MeSH$$aAxons: metabolism
000169351 650_2 $$2MeSH$$aNeurons: metabolism
000169351 650_2 $$2MeSH$$aDrosophila Proteins: genetics
000169351 650_2 $$2MeSH$$aDrosophila Proteins: metabolism
000169351 7001_ $$aKawai, Hiroki$$b1
000169351 7001_ $$aMaki, Ryuto$$b2
000169351 7001_ $$aOsaka, Jiro$$b3
000169351 7001_ $$aHakeda-Suzuki, Satoko$$b4
000169351 7001_ $$aNagai, Yoshitaka$$b5
000169351 7001_ $$0P:(DE-2719)9000534$$aDoubková, Karolína$$b6$$udzne
000169351 7001_ $$aUehara, Tomoko$$b7
000169351 7001_ $$aWatanabe, Kenji$$b8
000169351 7001_ $$aKosaki, Kenjiro$$b9
000169351 7001_ $$aSuzuki, Takashi$$b10
000169351 7001_ $$0P:(DE-2719)2810271$$aTavosanis, Gaia$$b11$$udzne
000169351 7001_ $$0P:(DE-2719)2810439$$aSugie, Atsushi$$b12$$udzne
000169351 773__ $$0PERI:(DE-600)1474816-2$$a10.1093/hmg/ddac307$$gp. ddac307$$n9$$p1524-1538$$tHuman molecular genetics$$v32$$x0964-6906$$y2023
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