% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Nitta:169351,
      author       = {Nitta, Yohei and Kawai, Hiroki and Maki, Ryuto and Osaka,
                      Jiro and Hakeda-Suzuki, Satoko and Nagai, Yoshitaka and
                      Doubková, Karolína and Uehara, Tomoko and Watanabe, Kenji
                      and Kosaki, Kenjiro and Suzuki, Takashi and Tavosanis, Gaia
                      and Sugie, Atsushi},
      title        = {{D}irect evaluation of neuroaxonal degeneration with the
                      causative genes of neurodegenerative diseases in drosophila
                      using the automated axon quantification system,
                      {M}e{DU}s{A}.},
      journal      = {Human molecular genetics},
      volume       = {32},
      number       = {9},
      issn         = {0964-6906},
      address      = {Oxford},
      publisher    = {Oxford Univ. Press},
      reportid     = {DZNE-2023-00126},
      pages        = {1524-1538},
      year         = {2023},
      abstract     = {Drosophila is an excellent model organism for studying
                      human neurodegenerative diseases (NDs). However, there is
                      still almost no experimental system that could directly
                      observe the degeneration of neurons and automatically
                      quantify axonal degeneration. In this study, we created
                      MeDUsA (a 'method for the quantification of degeneration
                      using fly axons'), a standalone executable computer program
                      based on Python that combines a pre-trained deep-learning
                      masking tool with an axon terminal counting tool. This
                      software automatically quantifies the number of retinal R7
                      axons in Drosophila from a confocal z-stack image series.
                      Using this software, we were able to directly demonstrate
                      that axons were degenerated by the representative causative
                      genes of NDs for the first time in Drosophila. The fly
                      retinal axon is an excellent experimental system that is
                      capable of mimicking the pathology of axonal degeneration in
                      human NDs. MeDUsA rapidly and accurately quantifies axons in
                      Drosophila photoreceptor neurons. It enables large-scale
                      research into axonal degeneration, including screening to
                      identify genes or drugs that mediate axonal toxicity caused
                      by ND proteins and diagnose the pathological significance of
                      novel variants of human genes in axons.},
      keywords     = {Animals / Humans / Drosophila: genetics / Drosophila:
                      metabolism / Neurodegenerative Diseases: metabolism / Axons:
                      metabolism / Neurons: metabolism / Drosophila Proteins:
                      genetics / Drosophila Proteins: metabolism / Drosophila
                      Proteins (NLM Chemicals)},
      cin          = {AG Tavosanis},
      ddc          = {570},
      cid          = {I:(DE-2719)1013018},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36611008},
      doi          = {10.1093/hmg/ddac307},
      url          = {https://pub.dzne.de/record/169351},
}