000194989 001__ 194989
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000194989 037__ $$aDZNE-2023-00176
000194989 041__ $$aEnglish
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000194989 1001_ $$00000-0003-4447-5043$$aWang, Yuhan$$b0
000194989 245__ $$aMultimodal mapping of cell types and projections in the central nucleus of the amygdala.
000194989 260__ $$aCambridge$$beLife Sciences Publications$$c2023
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000194989 520__ $$a The central nucleus of the amygdala (CEA) is a brain region that integrates external and internal sensory information and executes innate and adaptive behaviors through distinct output pathways. Despite its complex functions, the diversity of molecularly defined neuronal types in the CEA and their contributions to major axonal projection targets have not been examined systematically. Here, we performed single-cell RNA-sequencing (scRNA-seq) to classify molecularly defined cell types in the CEA and identified marker genes to map the location of these neuronal types using expansion-assisted iterative fluorescence in situ hybridization (EASI-FISH). We developed new methods to integrate EASI-FISH with 5-plex retrograde axonal labeling to determine the spatial, morphological, and connectivity properties of ~30,000 molecularly defined CEA neurons. Our study revealed spatiomolecular organization of the CEA, with medial and lateral CEA associated with distinct molecularly defined cell families. We also found a long-range axon projection network from the CEA, where target regions receive inputs from multiple molecularly defined cell types. Axon collateralization was found primarily among projections to hindbrain targets, which are distinct from forebrain projections. This resource reports marker gene combinations for molecularly defined cell types and axon-projection types, which will be useful for selective interrogation of these neuronal populations to study their contributions to the diverse functions of the CEA.
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000194989 650_2 $$2MeSH$$aCentral Amygdaloid Nucleus: physiology
000194989 650_2 $$2MeSH$$aIn Situ Hybridization, Fluorescence
000194989 650_2 $$2MeSH$$aNeurons: physiology
000194989 650_2 $$2MeSH$$aAxons
000194989 650_2 $$2MeSH$$aNeural Pathways: metabolism
000194989 650_7 $$2Other$$aamygdala
000194989 650_7 $$2Other$$amouse
000194989 650_7 $$2Other$$aneuroscience
000194989 650_7 $$2Other$$afluorescent in situ hybridization
000194989 650_7 $$2Other$$atranscriptomics
000194989 7001_ $$0P:(DE-2719)9001056$$aKrabbe, Sabine$$b1$$udzne
000194989 7001_ $$aEddison, Mark$$b2
000194989 7001_ $$aHenry, Fredrick E$$b3
000194989 7001_ $$aFleishman, Greg$$b4
000194989 7001_ $$00000-0002-0624-3789$$aLemire, Andrew L$$b5
000194989 7001_ $$aWang, Lihua$$b6
000194989 7001_ $$00000-0001-8396-1533$$aKorff, Wyatt$$b7
000194989 7001_ $$00000-0002-2568-2365$$aTillberg, Paul W$$b8
000194989 7001_ $$aLüthi, Andreas$$b9
000194989 7001_ $$00000-0002-0835-444X$$aSternson, Scott$$b10
000194989 773__ $$0PERI:(DE-600)2687154-3$$a10.7554/eLife.84262$$gVol. 12, p. e84262$$pe84262$$teLife$$v12$$x2050-084X$$y2023
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