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@ARTICLE{Wang:194989,
      author       = {Wang, Yuhan and Krabbe, Sabine and Eddison, Mark and Henry,
                      Fredrick E and Fleishman, Greg and Lemire, Andrew L and
                      Wang, Lihua and Korff, Wyatt and Tillberg, Paul W and
                      Lüthi, Andreas and Sternson, Scott},
      title        = {{M}ultimodal mapping of cell types and projections in the
                      central nucleus of the amygdala.},
      journal      = {eLife},
      volume       = {12},
      issn         = {2050-084X},
      address      = {Cambridge},
      publisher    = {eLife Sciences Publications},
      reportid     = {DZNE-2023-00176},
      pages        = {e84262},
      year         = {2023},
      abstract     = {The central nucleus of the amygdala (CEA) is a brain region
                      that integrates external and internal sensory information
                      and executes innate and adaptive behaviors through distinct
                      output pathways. Despite its complex functions, the
                      diversity of molecularly defined neuronal types in the CEA
                      and their contributions to major axonal projection targets
                      have not been examined systematically. Here, we performed
                      single-cell RNA-sequencing (scRNA-seq) to classify
                      molecularly defined cell types in the CEA and identified
                      marker genes to map the location of these neuronal types
                      using expansion-assisted iterative fluorescence in situ
                      hybridization (EASI-FISH). We developed new methods to
                      integrate EASI-FISH with 5-plex retrograde axonal labeling
                      to determine the spatial, morphological, and connectivity
                      properties of ~30,000 molecularly defined CEA neurons. Our
                      study revealed spatiomolecular organization of the CEA, with
                      medial and lateral CEA associated with distinct molecularly
                      defined cell families. We also found a long-range axon
                      projection network from the CEA, where target regions
                      receive inputs from multiple molecularly defined cell types.
                      Axon collateralization was found primarily among projections
                      to hindbrain targets, which are distinct from forebrain
                      projections. This resource reports marker gene combinations
                      for molecularly defined cell types and axon-projection
                      types, which will be useful for selective interrogation of
                      these neuronal populations to study their contributions to
                      the diverse functions of the CEA.},
      keywords     = {Central Amygdaloid Nucleus: physiology / In Situ
                      Hybridization, Fluorescence / Neurons: physiology / Axons /
                      Neural Pathways: metabolism / amygdala (Other) / mouse
                      (Other) / neuroscience (Other) / fluorescent in situ
                      hybridization (Other) / transcriptomics (Other)},
      cin          = {AG Krabbe},
      ddc          = {600},
      cid          = {I:(DE-2719)5000059},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pmc          = {pmc:PMC9977318},
      pubmed       = {pmid:36661218},
      doi          = {10.7554/eLife.84262},
      url          = {https://pub.dzne.de/record/194989},
}