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001     194989
005     20240112171806.0
024 7 _ |a pmc:PMC9977318
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037 _ _ |a DZNE-2023-00176
041 _ _ |a English
082 _ _ |a 600
100 1 _ |a Wang, Yuhan
|0 0000-0003-4447-5043
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245 _ _ |a Multimodal mapping of cell types and projections in the central nucleus of the amygdala.
260 _ _ |a Cambridge
|c 2023
|b eLife Sciences Publications
336 7 _ |a article
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336 7 _ |a ARTICLE
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520 _ _ |a The central nucleus of the amygdala (CEA) is a brain region that integrates external and internal sensory information and executes innate and adaptive behaviors through distinct output pathways. Despite its complex functions, the diversity of molecularly defined neuronal types in the CEA and their contributions to major axonal projection targets have not been examined systematically. Here, we performed single-cell RNA-sequencing (scRNA-seq) to classify molecularly defined cell types in the CEA and identified marker genes to map the location of these neuronal types using expansion-assisted iterative fluorescence in situ hybridization (EASI-FISH). We developed new methods to integrate EASI-FISH with 5-plex retrograde axonal labeling to determine the spatial, morphological, and connectivity properties of ~30,000 molecularly defined CEA neurons. Our study revealed spatiomolecular organization of the CEA, with medial and lateral CEA associated with distinct molecularly defined cell families. We also found a long-range axon projection network from the CEA, where target regions receive inputs from multiple molecularly defined cell types. Axon collateralization was found primarily among projections to hindbrain targets, which are distinct from forebrain projections. This resource reports marker gene combinations for molecularly defined cell types and axon-projection types, which will be useful for selective interrogation of these neuronal populations to study their contributions to the diverse functions of the CEA.
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650 _ 2 |a Central Amygdaloid Nucleus: physiology
|2 MeSH
650 _ 2 |a In Situ Hybridization, Fluorescence
|2 MeSH
650 _ 2 |a Neurons: physiology
|2 MeSH
650 _ 2 |a Axons
|2 MeSH
650 _ 2 |a Neural Pathways: metabolism
|2 MeSH
650 _ 7 |a amygdala
|2 Other
650 _ 7 |a mouse
|2 Other
650 _ 7 |a neuroscience
|2 Other
650 _ 7 |a fluorescent in situ hybridization
|2 Other
650 _ 7 |a transcriptomics
|2 Other
700 1 _ |a Krabbe, Sabine
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700 1 _ |a Eddison, Mark
|b 2
700 1 _ |a Henry, Fredrick E
|b 3
700 1 _ |a Fleishman, Greg
|b 4
700 1 _ |a Lemire, Andrew L
|0 0000-0002-0624-3789
|b 5
700 1 _ |a Wang, Lihua
|b 6
700 1 _ |a Korff, Wyatt
|0 0000-0001-8396-1533
|b 7
700 1 _ |a Tillberg, Paul W
|0 0000-0002-2568-2365
|b 8
700 1 _ |a Lüthi, Andreas
|b 9
700 1 _ |a Sternson, Scott
|0 0000-0002-0835-444X
|b 10
773 _ _ |a 10.7554/eLife.84262
|g Vol. 12, p. e84262
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856 4 _ |u https://pub.dzne.de/record/194989/files/DZNE-2023-00176.pdf
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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