000249926 001__ 249926
000249926 005__ 20240118153846.0
000249926 037__ $$aDZNE-2023-00241
000249926 1001_ $$0P:(DE-2719)2810938$$aMüller, Stephan A$$b0$$eFirst author$$udzne
000249926 245__ $$aDataset: Quantitative secretome analysis using improved secretome-protein-enrichment-with-click-sugars method (iSPECS) establishes the cell type-resolved mouse brain glyco-secretome
000249926 260__ $$bPRoteomics IDEntifications Database$$c2020
000249926 3367_ $$2BibTeX$$aMISC
000249926 3367_ $$0PUB:(DE-HGF)32$$2PUB:(DE-HGF)$$aDataset$$bdataset$$mdataset$$s1705588708_9223
000249926 3367_ $$026$$2EndNote$$aChart or Table
000249926 3367_ $$2DataCite$$aDataset
000249926 3367_ $$2ORCID$$aDATA_SET
000249926 3367_ $$2DINI$$aResearchData
000249926 520__ $$aTo understand how cells communicate with each other, it is essential to define the cellular secretome, a collection of proteins including soluble secreted, unconventionally secreted and proteolytically-shed proteins. Quantitative methodologies to decipher the secretome are challenging, due to the requirement of large cell numbers and abundant serum proteins that interfere with the detection of low-abundant cellular secretome proteins. Here, we miniaturized secretome analysis by developing the improved secretome-protein-enrichment-with-click-sugars method (iSPECS), which identifies the glyco-secretome. We applied this method to provide a cell type-resolved mouse brain glyco-secretome resource. Our data show that a surprisingly high number of secreted proteins are generated by ectodomain shedding in a cell type-specific manner. Two examples are neuronally secreted ADAM22 and CD200, which we identified as new substrates of the Alzheimer-linked protease BACE1. Taken together, iSPECS and the brain glyco-secretome resource can be exploited for a wide range of applications to study protein secretion and shedding.
000249926 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000249926 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan$$b1$$eLast author$$udzne
000249926 7870_ $$0DZNE-2021-00213$$aTüshaus, Johanna et.al.$$dHoboken, NJ [u.a.] : Wiley, 2020$$iRelatedTo$$r$$tAn optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome.
000249926 8564_ $$uhttps://www.ebi.ac.uk/pride/archive/projects/PXD018171
000249926 909CO $$ooai:pub.dzne.de:249926$$pVDB
000249926 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810938$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000249926 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2181459$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000249926 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000249926 9141_ $$y2020
000249926 9201_ $$0I:(DE-2719)1110006$$kAG Lichtenthaler$$lNeuroproteomics$$x0
000249926 980__ $$adataset
000249926 980__ $$aVDB
000249926 980__ $$aI:(DE-2719)1110006
000249926 980__ $$aUNRESTRICTED