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@MISC{Mller:249926,
      author       = {Müller, Stephan A and Lichtenthaler, Stefan},
      title        = {{D}ataset: {Q}uantitative secretome analysis using improved
                      secretome-protein-enrichment-with-click-sugars method
                      (i{SPECS}) establishes the cell type-resolved mouse brain
                      glyco-secretome},
      publisher    = {PRoteomics IDEntifications Database},
      reportid     = {DZNE-2023-00241},
      year         = {2020},
      abstract     = {To understand how cells communicate with each other, it is
                      essential to define the cellular secretome, a collection of
                      proteins including soluble secreted, unconventionally
                      secreted and proteolytically-shed proteins. Quantitative
                      methodologies to decipher the secretome are challenging, due
                      to the requirement of large cell numbers and abundant serum
                      proteins that interfere with the detection of low-abundant
                      cellular secretome proteins. Here, we miniaturized secretome
                      analysis by developing the improved
                      secretome-protein-enrichment-with-click-sugars method
                      (iSPECS), which identifies the glyco-secretome. We applied
                      this method to provide a cell type-resolved mouse brain
                      glyco-secretome resource. Our data show that a surprisingly
                      high number of secreted proteins are generated by ectodomain
                      shedding in a cell type-specific manner. Two examples are
                      neuronally secreted ADAM22 and CD200, which we identified as
                      new substrates of the Alzheimer-linked protease BACE1. Taken
                      together, iSPECS and the brain glyco-secretome resource can
                      be exploited for a wide range of applications to study
                      protein secretion and shedding.},
      cin          = {AG Lichtenthaler},
      cid          = {I:(DE-2719)1110006},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)32},
      url          = {https://pub.dzne.de/record/249926},
}