TY  - JOUR
AU  - Quesnel, Agathe
AU  - Coles, Nathan
AU  - Angione, Claudio
AU  - Dey, Priyanka
AU  - Polvikoski, Tuomo M
AU  - Outeiro, Tiago F
AU  - Islam, Meez
AU  - Khundakar, Ahmad A
AU  - Filippou, Panagiota S
TI  - Glycosylation spectral signatures for glioma grade discrimination using Raman spectroscopy.
JO  - BMC cancer
VL  - 23
IS  - 1
SN  - 1471-2407
CY  - Heidelberg
PB  - Springer
M1  - DZNE-2023-00297
SP  - 174
PY  - 2023
N1  - CC BY
AB  - Gliomas are the most common brain tumours with the high-grade glioblastoma representing the most aggressive and lethal form. Currently, there is a lack of specific glioma biomarkers that would aid tumour subtyping and minimally invasive early diagnosis. Aberrant glycosylation is an important post-translational modification in cancer and is implicated in glioma progression. Raman spectroscopy (RS), a vibrational spectroscopic label-free technique, has already shown promise in cancer diagnostics.RS was combined with machine learning to discriminate glioma grades. Raman spectral signatures of glycosylation patterns were used in serum samples and fixed tissue biopsy samples, as well as in single cells and spheroids.Glioma grades in fixed tissue patient samples and serum were discriminated with high accuracy. Discrimination between higher malignant glioma grades (III and IV) was achieved with high accuracy in tissue, serum, and cellular models using single cells and spheroids. Biomolecular changes were assigned to alterations in glycosylation corroborated by analysing glycan standards and other changes such as carotenoid antioxidant content.RS combined with machine learning could pave the way for more objective and less invasive grading of glioma patients, serving as a useful tool to facilitate glioma diagnosis and delineate biomolecular glioma progression changes.
KW  - Humans
KW  - Spectrum Analysis, Raman: methods
KW  - Glycosylation
KW  - Glioma: pathology
KW  - Brain Neoplasms: pathology
KW  - Glioblastoma: pathology
KW  - Neoplasm Grading
KW  - Biomolecular signatures (Other)
KW  - Diagnosis (Other)
KW  - Glioblastoma (Other)
KW  - Gliomas (Other)
KW  - Glycosylation (Other)
KW  - Raman spectroscopy (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:36809974
C2  - pmc:PMC9942363
DO  - DOI:10.1186/s12885-023-10588-w
UR  - https://pub.dzne.de/record/255496
ER  -