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@ARTICLE{Nebeling:256466,
      author       = {Nebeling, Felix Christopher and Poll, Stefanie and Justus,
                      Lena Christine and Steffen, Julia and Keppler, Kevin and
                      Mittag, Manuel and Fuhrmann, Martin},
      title        = {{M}icroglial motility is modulated by neuronal activity and
                      correlates with dendritic spine plasticity in the
                      hippocampus of awake mice.},
      journal      = {eLife},
      volume       = {12},
      issn         = {2050-084X},
      address      = {Cambridge},
      publisher    = {eLife Sciences Publications},
      reportid     = {DZNE-2023-00328},
      pages        = {e83176},
      year         = {2023},
      note         = {CC BY},
      abstract     = {Microglia, the resident immune cells of the brain, play a
                      complex role in health and disease. They actively survey the
                      brain parenchyma by physically interacting with other cells
                      and structurally shaping the brain. Yet, the mechanisms
                      underlying microglial motility and significance for synapse
                      stability, especially in the hippocampus during adulthood,
                      remain widely unresolved. Here, we investigated the effect
                      of neuronal activity on microglial motility and the
                      implications for the formation and survival of dendritic
                      spines on hippocampal CA1 neurons in vivo. We used
                      repetitive two-photon in vivo imaging in the hippocampus of
                      awake and anesthetized mice to simultaneously study the
                      motility of microglia and their interaction with dendritic
                      spines. We found that CA3 to CA1 input is sufficient to
                      modulate microglial process motility. Simultaneously, more
                      dendritic spines emerged in mice after awake compared to
                      anesthetized imaging. Interestingly, the rate of microglial
                      contacts with individual dendritic spines and dendrites was
                      associated with the stability, removal, and emergence of
                      dendritic spines. These results suggest that microglia might
                      sense neuronal activity via neurotransmitter release and
                      actively participate in synaptic rewiring of the hippocampal
                      neural network during adulthood. Further, this study has
                      profound relevance for hippocampal learning and memory
                      processes.},
      keywords     = {Mice / Animals / Microglia: physiology / Dendritic Spines:
                      physiology / Wakefulness / Hippocampus: physiology / Neurons
                      / Neuronal Plasticity: physiology / chemogenetics (Other) /
                      dendritic spines (Other) / hippocampus (Other) / microglia
                      (Other) / mouse (Other) / neuroscience (Other) / two-photon
                      (Other)},
      cin          = {AG Fuhrmann / LMF},
      ddc          = {600},
      cid          = {I:(DE-2719)1011004 / I:(DE-2719)1040180},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      experiment   = {EXP:(DE-2719)LMF-20190308},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:36749020},
      pmc          = {pmc:PMC9946443},
      doi          = {10.7554/eLife.83176},
      url          = {https://pub.dzne.de/record/256466},
}