%0 Journal Article
%A Antoniou, Anna
%A Auderset, Loic
%A Kaurani, Lalit
%A Sebastian, Eva
%A Zeng, Yuzhou
%A Allahham, Maria
%A Cases-Cunillera, Silvia
%A Schoch, Susanne
%A Gründemann, Jan
%A Fischer, Andre
%A Schneider, Anja
%T Neuronal extracellular vesicles and associated microRNAs induce circuit connectivity downstream BDNF.
%J Cell reports
%V 42
%N 2
%@ 2211-1247
%C [New York, NY]
%I Elsevier
%M DZNE-2023-00329
%P 112063
%D 2023
%Z CC BY
%X Extracellular vesicles (EVs) have emerged as mediators of cellular communication, in part via the delivery of associated microRNAs (miRNAs), small non-coding RNAs that regulate gene expression. We show that brain-derived neurotrophic factor (BDNF) mediates the sorting of miR-132-5p, miR-218-5p, and miR-690 in neuron-derived EVs. BDNF-induced EVs in turn increase excitatory synapse formation in recipient hippocampal neurons, which is dependent on the inter-neuronal delivery of these miRNAs. Transcriptomic analysis further indicates the differential expression of developmental and synaptogenesis-related genes by BDNF-induced EVs, many of which are predicted targets of miR-132-5p, miR-218-5p, and miR-690. Furthermore, BDNF-induced EVs up-regulate synaptic vesicle (SV) clustering in a transmissible manner, thereby increasing synaptic transmission and synchronous neuronal activity. As BDNF and EV-miRNAs miR-218 and miR-132 were previously implicated in neuropsychiatric disorders such as anxiety and depression, our results contribute to a better understanding of disorders characterized by aberrant neural circuit connectivity.
%K MicroRNAs: genetics
%K MicroRNAs: metabolism
%K Brain-Derived Neurotrophic Factor: metabolism
%K Neurons: metabolism
%K Extracellular Vesicles: metabolism
%K MicroRNAs (NLM Chemicals)
%K BDNF (Other)
%K CP: Neuroscience (Other)
%K exosome (Other)
%K extracellular vesicle (Other)
%K microRNA (Other)
%K network connectivity (Other)
%K neural circuit (Other)
%K synapse (Other)
%K synapse clustering (Other)
%K Brain-Derived Neurotrophic Factor (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:36753414
%R 10.1016/j.celrep.2023.112063
%U https://pub.dzne.de/record/256467